From Tol2Kit
Revision as of 17:48, 7 June 2007 by Chi-bin (talk | contribs)

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>pDestTol2pA2
CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT
TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC
TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT
GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA
AAAACCGTCTATCAGGGCGATGGCCCACGCTGGCTAAGAACTCATCAGCC
TCCCCGGTCCATCTACCCACGTACCAATGCACCAATTGGCCACAATGACG
GCTACTACATGGTGCCATTCCTTCCTCTTTATAGGAATGGAGACTACCTC
CTGTCCAACAAGGCTCTTGGATACGAGTACGCCTACCTGTTGGACCCAGG
TCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGAATAT
CTGTTCAGACACCCATATCCACTCTGTTCCACACAGGTCAGAGGTTTGTC
CAGGAGTTCTTGACAGAGGTGTAAAAAGTACTCAAAAATTTTACTCAAGT
GAAAGTACAAGTACTTAGGGAAAATTTTACTCAATTAAAAGTAAAAGTAT
CTGGCTAGAATCTTACTTGAGTAAAAGTAAAAAAGTACTCCATTAAAATT
GTACTTGAGTATTAAGGAAGTAAAAGTAAAAGCAAGAAAGAAAACTAGAG
ATTCTTGTTTAAGCTTTTAATCTCAAAAAACATTAAATGAAATGCATACA
AGGTTTTATCCTGCTTTAGAACTGTTTGTATTTAATTATCAAACTATAAG
ACAGACAATCTAATGCCAGTACACGCTACTCAAAGTTGTAAAACCTCAGA
TTTAACTTCAGTAGAAGCTGATTCTCAAAATTGTTAGTGTCAAGCCTAGC
TCTTTTGGGGCTGAAAAGCAATCCTGCAGTGCTGAAAAGCCTCTCACAGG
CAGCCGATGCGGGAAGAGGTGTATTAGTCTTGATAGAGAGGCTGCAAATA
GCAGGAAACGTGAGCAGAGACTCCCTGGTGTCTGAAACACAGGCCAGATG
GGCCCTCGAGCAGGAAACAGCTATGACCATGATTACGCCAAGCTATCAAC
TTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATCAATA
TATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAAAACA
CAACATATCCAGTCACTATGGTCGACCTGCAGACTGGCTGTGTATAAGGG
AGCCTGACATTTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATG
TCATTTTCGCGGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGAC
CGGCACACTGGCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGA
TATGCACCACCGGGTAAAGTTCACGGGGGACTTTATCTGACAGCAGACGT
GCACTGGCCAGGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATC
ACTCTGTACATCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGT
AAACCTTAAACTGCATTTCACCAGCCCCTGTTCTCGTCGGCAAAGGAGCC
GTTCATTTCAATAAACCGGGCGACCTCAGCCATCCCTTCCTGATTTTCCG
CTTTCCAGCGTTCGGCACGCAGACGACGGGCTTCATTCTGCATGGTTGTG
CTTACCGAACCGGAGATATTGACATCATATATGCCTTGAGCAACTGATAG
CTGTCGCTGTCAACTGTCACTGTAATACGCTGCTTCATAGCATACCTCTT
TTTGACATACTTCGGGTATACATATCAGTATATATTCTTATACCGCAAAA
ATCAGCGCGCAAATACGCATACTGTTATCTGGCTTTTAGTAAGCCGGATC
CTCTAGATTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCA
TTAAGCATTCTGCCGACATGGAAGCCATCACAAACGGCATGATGAACCTG
AATCGCCAGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCA
TGGTGAAAACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCA
AAACTGGTGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTC
AATAAACCCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACAT
CTTGCGAATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTC
CAGAGCGATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGG
GTGAACACTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGA
ATTCCGGATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGA
TAAAACTTGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATC
CAGCTGAACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCT
CAAAATGTTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCA
GTGATTTTTTTCTCCATTTTAGCTTCCTTAGCTCCTGAAAATCTCGACGG
ATCCTAACTCAAAATCCACACATTATACGAGCCGGAAGCATAAAGTGTAA
AGCCTGGGGGTGCCTAATGCGGCCGCCATAGTGACTGGATATGTTGTGTT
TTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATT
GATATTTATATCATTTTACGTTTCTCGTTCAACTTTATTATACATAGTTG
ATAATTCACTGGCCGTCGTTTTACGGTACCATCGATGATGATCCAGACAT
GATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAA
AAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACC
ATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTAT
GTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAGCAAGTAAAACC
TCTACAAATGTGGTATGGCTGATTATGATCCTCTAGATCAGATCTGCGAA
GATACGGCCACGGGTGCTCTTGATCCTGTGGCTGATTTTGGACTGTGCTG
CTCGCAGCTGCTGATGAATCACATACTTCCTCCATTTTCTTCCACTGATT
GACTGTTATAATTTCCCTAATTTCCAGGTCAAGGTGCTGTGCATTGTGGT
AATAGATGTGACATGACGTCACTTCCAAAGGACCAATGAACATGTCTGAC
CAATTTCATATAATGTGAAAACGATTTTCATAGGCAGAATAAATAACATT
TAAATTAAACTGGGCATCAGCGCAATTCAATTGGTTTGGTAATAGCAAGG
GAAAATAGAATGAAGTGATCTCCAAAAAATAAGTACTTTTTGACTGTAAA
TAAAATTGTAAGGAGTAAAAAGTACTTTTTTTTCTAAAAAAATGTAATTA
AGTAAAAGTAAAAGTATTGATTTTTAATTGTACTCAAGTAAAGTAAAAAT
CCCCAAAAATAATACTTAAGTACAGTAATCAAGTAAAATTACTCAAGTAC
TTTACACCTCTGGTTCTTGACCCCCTACCTTCAGCAAGCCCAGCAGATCC
ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC
TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT
CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG
AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT
TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC
CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT
TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC
GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC
ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA
AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG
CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC
CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG
TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA
ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT
AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA
GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCC
GGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA
GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC
ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG
AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT
ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGT
TCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA
GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT
CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAG
CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG
TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACG
TTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG
GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCC
CATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA
GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCAT
AATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA
GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT
CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTA
AAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT
CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT
GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACA
GGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG
AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT
ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA
ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG
>pBSII_SK+_5’_of_DraIII
CCACCTAAATTGTAAGCGTTAATATTTTGTTAAAATTCGCGTTAAATTTT
TGTTAAATCAGCTCATTTTTTAACCAATAGGCCGAAATCGGCAAAATCCC
TTATAAATCAAAAGAATAGACCGAGATAGGGTTGAGTGTTGTTCCAGTTT
GGAACAAGAGTCCACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGA
AAAACCGTCTATCAGGGCGATGGCCCAC
>f1_(+)_ori_fragment
GTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCC
ACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAA
>tyrosinase_genomic_from_AB010101
GCTGGCTAAGAACTCATCAGCCTCCCCGGTCCATCTACCCACGTACCAAT
GCACCAATTGGCCACAATGACGGCTACTACATGGTGCCATTCCTTCCTCT
TTATAGGAATGGAGACTACCTCCTGTCCAACAAGGCTCTTGGATACGAGT
ACGCCTACCTGTTGGACCCAGGTCATTGCACAACACCAGAAATGCCCTCT
GATCTGCAAAAGACGTGAATATCTGTTCAGACACCCATATCCACTCTGTT
CCACACAGGTCAGAGGTTTGTCCAGGAGTTCTTGAC
>Tyro_frag_ex4-in4-ex5
CAGGTCATTGCACAACACCAGAAATGCCCTCTGATCTGCAAAAGACGTGA
ATATCTGTTCAGACACCCATATCCACTCTGTTCCACACAGG
>3'_TIR
TACTTTTTACACCTCTG
>536_bp_of_Tol2_3'_end
ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT
ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC
CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG
CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT
CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC
TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT
GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGAATC
TCTAGTTTTCTTTCTTGCTTTTACTTTTACTTCCTTAATACTCAAGTACA
ATTTTAATGGAGTACTTTTTTACTTTTACTCAAGTAAGATTCTAGCCAGA
TACTTTTACTTTTAATTGAGTAAAATTTTCCCTAAGTACTTGTACTTTCA
CTTGAGTAAAATTTTTGAGTACTTTTTACACCTCTG
>Tol2_exon4_136-482
ATCTGGCCTGTGTTTCAGACACCAGGGAGTCTCTGCTCACGTTTCCTGCT
ATTTGCAGCCTCTCTATCAAGACTAATACACCTCTTCCCGCATCGGCTGC
CTGTGAGAGGCTTTTCAGCACTGCAGGATTGCTTTTCAGCCCCAAAAGAG
CTAGGCTTGACACTAACAATTTTGAGAATCAGCTTCTACTGAAGTTAAAT
CTGAGGTTTTACAACTTTGAGTAGCGTGTACTGGCATTAGATTGTCTGTC
TTATAGTTTGATAATTAAATACAAACAGTTCTAAAGCAGGATAAAACCTT
GTATGCATTTCATTTAATGTTTTTTGAGATTAAAAGCTTAAACAAGA
>M13R
GGAAACAGCTATGACCATGA
>attB4/L4/R4_shared
CAACTTTGTATAGAAAAGTTG
>attR4
CAACTTTGTATAGAAAAGTTGAACGAGAAACGTAAAATGATATAAATATC
AATATATTAAATTAGATTTTGCATAAAAAACAGACTACATAATACTGTAA
AACACAACATATCCAGTCACTATGG
>ccdB
TTATATTCCCCAGAACATCAGGTTAATGGCGTTTTTGATGTCATTTTCGC
GGTGGCTGAGATCAGCCACTTCTTCCCCGATAACGGAGACCGGCACACTG
GCCATATCGGTGGTCATCATGCGCCAGCTTTCATCCCCGATATGCACCAC
CGGGTAAAGTTCACGGGAGACTTTATCTGACAGCAGACGTGCACTGGCCA
GGGGGATCACCATCCGTCGCCCGGGCGTGTCAATAATATCACTCTGTACA
TCCACAAACAGACGATAACGGCTCTCTCTTTTATAGGTGTAAACCTTAAA
CTGCAT
>ccdB_from_pDEST_R4-R3_(1_mutation)
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTT
TGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGA
TCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCCCCCGTGAACTT
TACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGA
TATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCA
GCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGA
ATATAA
>cmR
TTACGCCCCGCCCTGCCACTCATCGCAGTACTGTTGTAATTCATTAAGCA
TTCTGCCGACATGGAAGCCATCACAGACGGCATGATGAACCTGAATCGCC
AGCGGCATCAGCACCTTGTCGCCTTGCGTATAATATTTGCCCATGGTGAA
AACGGGGGCGAAGAAGTTGTCCATATTGGCCACGTTTAAATCAAAACTGG
TGAAACTCACCCAGGGATTGGCTGAGACGAAAAACATATTCTCAATAAAC
CCTTTAGGGAAATAGGCCAGGTTTTCACCGTAACACGCCACATCTTGCGA
ATATATGTGTAGAAACTGCCGGAAATCGTCGTGGTATTCACTCCAGAGCG
ATGAAAACGTTTCAGTTTGCTCATGGAAAACGGTGTAACAAGGGTGAACA
CTATCCCATATCACCAGCTCACCGTCTTTCATTGCCATACGGAATTCCGG
ATGAGCATTCATCAGGCGGGCAAGAATGTGAATAAAGGCCGGATAAAACT
TGTGCTTATTTTTCTTTACGGTCTTTAAAAAGGCCGTAATATCCAGCTGA
ACGGTCTGGTTATAGGTACATTGAGCAACTGACTGAAATGCCTCAAAATG
TTCTTTACGATGCCATTGGGATATATCAACGGTGGTATATCCAGTGATTT
TTTTCTCCAT
>attR3
CCATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTT
TATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCT
CGTTCAACTTTATTATACATAGTTG
>attB3/L3/R3_shared
CAACTTTATTATACA
>M13_-20
GTAAAACGACGGCCAGTG
>SV40_late_polyA
GATCCAGACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAG
AATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCTT
TATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGC
ATTCATTTTATGTTTCAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTAAAG
CAAGTAAAACCTCTACAAATGTGGTATGGCTGATTAT
>Tol2_exon1,_bp1-335_(of_461)
ATTTTTTGGAGATCACTTCATTCTATTTTCCCTTGCTATTACCAAACCAA
TTGAATTGCGCTGATGCCCAGTTTAATTTAAATGTTATTTATTCTGCCTA
TGAAAATCGTTTTCACATTATATGAAATTGGTCAGACATGTTCATTGGTC
CTTTGGAAGTGACGTCATGTCACATCTATTACCACAATGCACAGCACCTT
GACCTGGAAATTAGGGAAATTATAACAGTCAATCAGTGGAAGAAAATGGA
GGAAGTATGTGATTCATCAGCAGCTGCGAGCAGCACAGTCCAAAATCAGC
CACAGGATCAAGAGCACCCGTGGCCGTATCTTCGC
>517_bp_of_Tol2_5'_end
CAGAGGTGTAAAGTACTTGAGTAATTTTACTTGATTACTGTACTTAAGTA
TTATTTTTGGGGATTTTTACTTTACTTGAGTACAATTAAAAATCAATACT
TTTACTTTTACTTAATTACATTTTTTTAGAAAAAAAAGTACTTTTTACTC
CTTACAATTTTATTTACAGTCAAAAAGTACTTATTTTTTGGAGATCACTT
CATTCTATTTTCCCTTGCTATTACCAAACCAATTGAATTGCGCTGATGCC
CAGTTTAATTTAAATGTTATTTATTCTGCCTATGAAAATCGTTTTCACAT
TATATGAAATTGGTCAGACATGTTCATTGGTCCTTTGGAAGTGACGTCAT
GTCACATCTATTACCACAATGCACAGCACCTTGACCTGGAAATTAGGGAA
ATTATAACAGTCAATCAGTGGAAGAAAATGGAGGAAGTATGTGATTCATC
AGCAGCTGCGAGCAGCACAGTCCAAAATCAGCCACAGGATCAAGAGCACC
CGTGGCCGTATCTTCGC
>5'_TIR
CAGAGGTGTAAAGTACTTG
>Tyro_frag_ex5
GATCTGCTGGGCTTGCTGAAGGTAGGGGGTCAAGAAC
>pBSII_SK+_3’_of_SpeI
ACTAGTTCTAGAGCGGCCGCCACCGCGGTGGAGCTCCAGCTTTTGTTCCC
TTTAGTGAGGGTTAATTGCGCGCTTGGCGTAATCATGGTCATAGCTGTTT
CCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGG
AAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACAT
TAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGC
CAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTAT
TGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTC
GGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCC
ACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCA
AAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGC
TCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGG
CGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTC
CCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCG
CCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGG
TATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGA
ACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTG
AGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGT
AACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAA
GTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCG
CTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCC
GGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCA
GATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTA
CGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTC
ATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATG
AAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTT
ACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGT
TCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGA
GGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCT
CACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAG
CGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTG
TTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACG
TTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATG
GCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCC
CATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCA
GAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCAT
AATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGA
GTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCT
CTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTA
AAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGAT
CTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACT
GATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACA
GGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTG
AATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTT
ATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAA
ATAGGGGTTCCGCGCACATTTCCCCGAAAAGTG
>T3_primer
AATTAACCCTCACTAAAGGG
>M13R
GGAAACAGCTATGACCATGA
>pUC_ori
TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCT
GGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGAC
GCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCG
TTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCT
TACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTC
ATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG
CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATC
CGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCAC
TGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGT
GCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGAC
AGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAG
TTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT
TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGA
TCCTTTGATCTTTTCTAC
>amp
ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATT
TTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATG
CTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAAC
AGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGAT
GAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACG
CCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG
GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGT
AAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCA
ACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTG
CACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCT
GAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAA
TGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT
TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACC
ACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTG
GAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGAT
GGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAC
TATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTA
AGCATTGG