From Tol2Kit
Construction details
pME-EGFPCAAX was made by PCR amplification of EGFP-CAAX, using primers to add att sites, followed by a BP reaction. EGFP-CAAX is EGFP with a C-terminal fusion of 21 amino acids of human Harvey Ras, encoding a prenylation signal. Sequencing confirms that all bases of the insert are correct.
Sequence
Annotated sequence, Genbank format:
pME-EGFPCAAX Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
pME-EGFPCAAX sequence
Crude map
Screenshot from Sequencher showing locations of components:
