PME-EGFP no stop

Construction details

pME-EGFP no stop was made by PCR amplification of the EGFP insert from a pCS2-based plasmid, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

This clone can be used to generate N-terminal EGFP fusions with a gene of interest placed in a 3' clone.

Sequence

Annotated sequence, Genbank format:
pME-EGFP no stop Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-EGFP no stop sequence

Crude map

Screenshot from Sequencher showing locations of components: