From Tol2Kit
Revision as of 04:06, 19 December 2007 by Chi-bin (talk | contribs) (New page: <pre> LOCUS 237.pME-MCS 2765 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, ...)

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LOCUS       237.pME-MCS        2765 bp ds-DNA     circular     18-DEC-2007
REFERENCE   1
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, 
			Parant JM, Yost HJ, Kanki JP, Chien CB.
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2 
			transposon transgenesis constructs.
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
  PUBMED    17937395
COMMENT     237.pME-MCS, Tol2kit v1.1
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     569..668
                     /label=attL1
                     /ApEinfo_fwdcolor=#3fd0ff
                     /ApEinfo_revcolor=#3fd0ff
     misc_recomb     890..989
                     /label=attL2
                     /ApEinfo_fwdcolor=#49a0ff
                     /ApEinfo_revcolor=#49a0ff
     misc_feature    649..662
                     /label=att1_shared
                     /ApEinfo_fwdcolor=#32a6cc
                     /ApEinfo_revcolor=#32a6cc
     misc_feature    896..911
                     /label=att2_shared
                     /ApEinfo_fwdcolor=#3a80cc
                     /ApEinfo_revcolor=#3a80cc
     misc_feature    695..861
                     /label=pBS MCS T7-T3
                     /ApEinfo_fwdcolor=#dddddd
                     /ApEinfo_revcolor=#dddddd
     rep_origin      2089..2762
                     /label=pUC ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     CDS             1159..1968
                     /label=kanR
                     /ApEinfo_fwdcolor=#ff7f3f
                     /ApEinfo_revcolor=#ff7f3f
     primer_bind     complement(1026..1046)
                     /label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     537..552
                     /label=M13F
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     670..685
                     /label=M13F(1)
                     /ApEinfo_label=M13F
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        complement(842..861)
                     /label=T3
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        complement(1003..1022)
                     /label=T7
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     695..717
                     /label=T7 primer
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     terminator      268..295
                     /label=T2
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
     terminator      427..470
                     /label=T1
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTTG TAAAACGACG GCCAGTGAGC GCGCGTAATA CGACTCACTA TAGGGCGAAT
      721 TGGGTACCGG GCCCCCCCTC GAGGTCGACG GTATCGATAA GCTTGATATC GAATTCCTGC
      781 AGCCCGGGGG ATCCACTAGT TCTAGAGCGG CCGCCACCGC GGTGGAGCTC CAGCTTTTGT
      841 TCCCTTTAGT GAGGGTTAAT TGCGCGCTTG GCGTAATCAT GGTCATAGCA CCCAGCTTTC
      901 TTGTACAAAG TTGGCATTAT AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC
      961 ACTATCAGTC AAAATAAAAT CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG
     1021 TATTACATGG TCATAGCTGT TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT
     1081 TACATTGCAC AAGATAAAAT AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC
     1141 AGTAATACAA GGGGTGTTAT GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT
     1201 TCCAACATGG ATGCTGATTT ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA
     1261 GGTGCGACAA TCTATCGCTT GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT
     1321 GGCAAAGGTA GCGTTGCCAA TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG
     1381 GAATTTATGC CTCTTCCGAC CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA
     1441 CTCACCACTG CGATCCCCGG AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA
     1501 GGTGAAAATA TTGTTGATGC GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT
     1561 TGTAATTGTC CTTTTAACAG CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG
     1621 AATAACGGTT TGGTTGATGC GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA
     1681 CAAGTCTGGA AAGAAATGCA TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT
     1741 GGTGATTTCT CACTTGATAA CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT
     1801 GTTGGACGAG TCGGAATCGC AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC
     1861 GGTGAGTTTT CTCCTTCATT ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT
     1921 GATATGAATA AATTGCAGTT TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT
     1981 AATTGGTTGT AACACTGGCA GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA
     2041 CCAAAATCCC TTAACGTGAG TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG
     2101 ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA
     2161 AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG
     2221 AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG
     2281 TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG
     2341 TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA
     2401 TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC
     2461 TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC
     2521 ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA
     2581 GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT
     2641 CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG
     2701 AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC
     2761 ATGTT     
//