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Construction details

pME-mCherry was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.


Annotated sequence, Genbank format:
pME-mCherry Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-mCherry sequence

Crude map

Screenshot from Sequencher showing locations of components: