From Tol2Kit
(New page: === Construction details === pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases ...) |
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=== Construction details === | === Construction details === | ||
pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. | pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. | ||
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| + | This clone can be used to generate N-terminal mCherry fusions with a gene of interest placed in a 3' clone. | ||
=== Crudely annotated sequence === | === Crudely annotated sequence === | ||
Revision as of 23:19, 2 November 2007
Construction details
pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
This clone can be used to generate N-terminal mCherry fusions with a gene of interest placed in a 3' clone.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-mCherry no stop sequence
Crude map
Screenshot from Sequencher showing locations of components:
