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(New page: === Construction details === pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases ...)
 
 
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pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction.  Sequencing confirms that all bases of the insert are correct.
 
pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction.  Sequencing confirms that all bases of the insert are correct.
  
=== Crudely annotated sequence ===
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This clone can be used to generate N-terminal mCherry fusions with a gene of interest placed in a 3' clone.
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=== Sequence ===
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Annotated sequence, Genbank format:<br>
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[[pME-mCherry no stop Genbank]]
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FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
[[pME-mCherry no stop sequence]]
 
[[pME-mCherry no stop sequence]]

Latest revision as of 03:49, 19 December 2007

Construction details

pME-mCherry no stop was made by PCR amplification of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

This clone can be used to generate N-terminal mCherry fusions with a gene of interest placed in a 3' clone.

Sequence

Annotated sequence, Genbank format:
pME-mCherry no stop Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
pME-mCherry no stop sequence

Crude map

Screenshot from Sequencher showing locations of components:
PME-mCherry no stop.png