From Tol2Kit
Revision as of 04:08, 19 December 2007 by Chi-bin (talk | contribs) (New page: <pre> LOCUS 456.pME-mCherry-no stop 3258 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, ...)

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LOCUS       456.pME-mCherry-no stop        3258 bp ds-DNA     circular     18-DEC-2007
REFERENCE   1
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, 
			Parant JM, Yost HJ, Kanki JP, Chien CB.
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2 
			transposon transgenesis constructs.
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
  PUBMED    17937395
COMMENT     456.pME-mCherry-no stop, Tol2kit v1.2
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     569..668
                     /label=attL1
                     /ApEinfo_fwdcolor=#3fd0ff
                     /ApEinfo_revcolor=#3fd0ff
     misc_recomb     1383..1482
                     /label=attL2
                     /ApEinfo_fwdcolor=#49a0ff
                     /ApEinfo_revcolor=#49a0ff
     misc_feature    649..662
                     /label=att1_shared
                     /ApEinfo_fwdcolor=#32a6cc
                     /ApEinfo_revcolor=#32a6cc
     misc_feature    1389..1404
                     /label=att2_shared
                     /ApEinfo_fwdcolor=#3a80cc
                     /ApEinfo_revcolor=#3a80cc
     CDS             674..1381
                     /label=mCherry (Svoboda)
                     /ApEinfo_fwdcolor=#dc143c
                     /ApEinfo_revcolor=#dc143c
     rep_origin      2582..3255
                     /label=pUC ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     CDS             1652..2461
                     /label=kanR
                     /ApEinfo_fwdcolor=#ff7f3f
                     /ApEinfo_revcolor=#ff7f3f
     primer_bind     complement(1519..1539)
                     /label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     537..552
                     /label=M13F
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        complement(1496..1515)
                     /label=T7
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     terminator      268..295
                     /label=T2
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
     terminator      427..470
                     /label=T1
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
      601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
      661 AGCAGGCTCC ACCATGGTGA GCAAGGGCGA GGAGGACAAC ATGGCCATCA TCAAGGAGTT
      721 CATGCGCTTC AAGGTGCACA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG
      781 CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG
      841 CGGCCCCCTG CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCATGTACG GCTCCAAGGC
      901 CTACGTGAAG CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT
      961 CAAGTGGGAG CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC
     1021 CTCCCTGCAG GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC
     1081 CGACGGCCCC GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCTCCG AGCGGATGTA
     1141 CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT CAAGCAGAGG CTGAAGCTGA AGGACGGCGG
     1201 CCACTACGAC GCCGAGGTCA AGACCACCTA CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG
     1261 CGCCTACAAC GTCAACATCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT
     1321 GGAACAGTAC GAGCGCGCCG AGGGCCGCCA CTCCACCGGC GGCATGGACG AGCTGTACAA
     1381 GCACCCAGCT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG
     1441 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT TGCCATCCAG CTGATATCCC
     1501 CTATAGTGAG TCGTATTACA TGGTCATAGC TGTTTCCTGG CAGCTCTGGC CCGTGTCTCA
     1561 AAATCTCTGA TGTTACATTG CACAAGATAA AATAATATCA TCATGAACAA TAAAACTGTC
     1621 TGCTTACATA AACAGTAATA CAAGGGGTGT TATGAGCCAT ATTCAACGGG AAACGTCGAG
     1681 GCCGCGATTA AATTCCAACA TGGATGCTGA TTTATATGGG TATAAATGGG CTCGCGATAA
     1741 TGTCGGGCAA TCAGGTGCGA CAATCTATCG CTTGTATGGG AAGCCCGATG CGCCAGAGTT
     1801 GTTTCTGAAA CATGGCAAAG GTAGCGTTGC CAATGATGTT ACAGATGAGA TGGTCAGACT
     1861 AAACTGGCTG ACGGAATTTA TGCCTCTTCC GACCATCAAG CATTTTATCC GTACTCCTGA
     1921 TGATGCATGG TTACTCACCA CTGCGATCCC CGGAAAAACA GCATTCCAGG TATTAGAAGA
     1981 ATATCCTGAT TCAGGTGAAA ATATTGTTGA TGCGCTGGCA GTGTTCCTGC GCCGGTTGCA
     2041 TTCGATTCCT GTTTGTAATT GTCCTTTTAA CAGCGATCGC GTATTTCGTC TCGCTCAGGC
     2101 GCAATCACGA ATGAATAACG GTTTGGTTGA TGCGAGTGAT TTTGATGACG AGCGTAATGG
     2161 CTGGCCTGTT GAACAAGTCT GGAAAGAAAT GCATAAACTT TTGCCATTCT CACCGGATTC
     2221 AGTCGTCACT CATGGTGATT TCTCACTTGA TAACCTTATT TTTGACGAGG GGAAATTAAT
     2281 AGGTTGTATT GATGTTGGAC GAGTCGGAAT CGCAGACCGA TACCAGGATC TTGCCATCCT
     2341 ATGGAACTGC CTCGGTGAGT TTTCTCCTTC ATTACAGAAA CGGCTTTTTC AAAAATATGG
     2401 TATTGATAAT CCTGATATGA ATAAATTGCA GTTTCATTTG ATGCTCGATG AGTTTTTCTA
     2461 ATCAGAATTG GTTAATTGGT TGTAACACTG GCAGAGCATT ACGCTGACTT GACGGGACGG
     2521 CGCAAGCTCA TGACCAAAAT CCCTTAACGT GAGTTACGCG TCGTTCCACT GAGCGTCAGA
     2581 CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG
     2641 CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC
     2701 AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTTCTTCT
     2761 AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC
     2821 TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT
     2881 GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG
     2941 CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT
     3001 ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG
     3061 GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG
     3121 TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG
     3181 GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG
     3241 GCCTTTTGCT CACATGTT    
//