P3E-IRES-EGFPCAAXpA

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[edit] Construction details

p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing finds a single bp change in the IRES (G481A), but this construct is functional.

[edit] Sequence

Annotated sequence, Genbank format:
p3E-IRES-EGFPCAAXpA Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p3E-IRES-EGFPCAAXpA sequence

[edit] Crude map

Screenshot from Sequencher showing locations of components:
Image:p3E-IRES-EGFPCAAXpA.png

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