From Tol2Kit
LOCUS 389.p3E-IRES-EGFPpA 4219 bp ds-DNA circular 18-DEC-2007
REFERENCE 1
AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
Parant JM, Yost HJ, Kanki JP, Chien CB.
TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2
transposon transgenesis constructs.
JOURNAL Developmental Dynamics 236(11):3088-99 (2007).
PUBMED 17937395
COMMENT 389.p3E-IRES-EGFPpA, Tol2kit v1.0
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_recomb 2328..2423
/label=attL3
/ApEinfo_fwdcolor=#5370ff
/ApEinfo_revcolor=#5370ff
misc_recomb 591..747
/label=attR2
/ApEinfo_fwdcolor=#49a0ff
/ApEinfo_revcolor=#49a0ff
misc_feature 730..745
/label=att2_shared
/ApEinfo_fwdcolor=#3a80cc
/ApEinfo_revcolor=#3a80cc
misc_feature 2328..2342
/label=att3_shared
/ApEinfo_fwdcolor=#4259cc
/ApEinfo_revcolor=#4259cc
polyA_signal 2131..2326
/label=SV40 late polyA (from pCS2+)
/ApEinfo_fwdcolor=#ffff7f
/ApEinfo_revcolor=#ffff7f
misc_structure 750..1334
/label=EMCV IRES(A7)
/ApEinfo_fwdcolor=#0000ff
/ApEinfo_revcolor=#0000ff
misc_feature 1326..1328
/label=EMCV IRES AUG 11
/ApEinfo_fwdcolor=#000099
/ApEinfo_revcolor=#000099
CDS 1338..2054
/label=EGFP
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=#00ff00
misc_structure 1529..1542
/label=EGFP-like chromophore
/ApEinfo_fwdcolor=#00cc00
/ApEinfo_revcolor=#00cc00
misc_feature 2055..2114
/label=pCS2 MCS fragment
/ApEinfo_fwdcolor=#dddddd
/ApEinfo_revcolor=#dddddd
rep_origin 3543..4216
/label=pUC ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
CDS 2613..3422
/label=kanR
/ApEinfo_fwdcolor=#ff7f3f
/ApEinfo_revcolor=#ff7f3f
primer_bind complement(2480..2500)
/label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind 537..552
/label=M13F
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(2457..2476)
/label=T7
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
terminator 268..295
/label=T2
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
terminator 427..470
/label=T1
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
ORIGIN
1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
541 AACGACGGCC AGTCTTAAGC TCGGGCCCTG CAGCTCTAGA GCTCGAATTC TACAGGTCAC
601 TAATACCATC TAAGTAGTTG ATTCATAGTG ACTGCATATG TTGTGTTTTA CAGTATTATG
661 TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT ATTTATATCA TTTTACGTTT
721 CTCGTTCAAC TTTCTTGTAC AAAGTGGCCG CCCCTCTCCC TCCCCCCCCC CTAACGTTAC
781 TGGCCGAAGC CGCTTGGAAT AAGGCCGGTG TGCGTTTGTC TATATGTTAT TTTCCACCAT
841 ATTGCCGTCT TTTGGCAATG TGAGGGCCCG GAAACCTGGC CCTGTCTTCT TGACGAGCAT
901 TCCTAGGGGT CTTTCCCCTC TCGCCAAAGG AATGCAAGGT CTGTTGAATG TCGTGAAGGA
961 AGCAGTTCCT CTGGAAGCTT CTTGAAGACA AACAACGTCT GTAGCGACCC TTTGCAGGCA
1021 GCGGAACCCC CCACCTGGCG ACAGGTGCCT CTGCGGCCAA AAGCCACGTG TATAAGATAC
1081 ACCTGCAAAG GCGGCACAAC CCCAGTGCCA CGTTGTGAGT TGGATAGTTG TGGAAAGAGT
1141 CAAATGGCTC TCCTCAAGCG TATTCAACAA GGGGCTGAAG GATGCCCAGA AGGTACCCCA
1201 TTGTATGGGA TCTGATCTGG GGCCTCGGTA CACATGCTTT ACATGTGTTT AGTCGAGGTT
1261 AAAAAAACGT CTAGGCCCCC CGAACCACGG GGACGTGGTT TTCCTTTGAA AAACACGATG
1321 ATAATATGGC CACAACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG GTGGTGCCCA
1381 TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC GGCGAGGGCG
1441 AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC GGCAAGCTGC
1501 CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC TTCAGCCGCT
1561 ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA GGCTACGTCC
1621 AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC GAGGTGAAGT
1681 TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC AAGGAGGACG
1741 GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC TATATCATGG
1801 CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC ATCGAGGACG
1861 GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC GGCCCCGTGC
1921 TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC CCCAACGAGA
1981 AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT CTCGGCATGG
2041 ACGAGCTGTA CAAGGGCGGT GGAAGATCTG GGAATTCAAG GCCTCTCGAG CCTCTAGATT
2101 CTGCAGCCCT ATAGTGAGTC GTATTACGTA GATCCAGACA TGATAAGATA CATTGATGAG
2161 TTTGGACAAA CCACAACTAG AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT
2221 GCTATTGCTT TATTTGTAAC CATTATAAGC TGCAATAAAC AAGTTAACAA CAACAATTGC
2281 ATTCATTTTA TGTTTCAGGT TCAGGGGGAG GTGTGGGAGG TTTTTTCCAA CTTTATTATA
2341 CAAAGTTGGC ATTATAAAAA AGCATTGCTT ATCAATTTGT TGCAACGAAC AGGTCACTAT
2401 CAGTCAAAAT AAAATCATTA TTTGGAGCTC CATGGTAGCG TTAACGCGGC CGCGATATCC
2461 CCTATAGTGA GTCGTATTAC ATGGTCATAG CTGTTTCCTG GCAGCTCTGG CCCGTGTCTC
2521 AAAATCTCTG ATGTTACATT GCACAAGATA AAAATATATC ATCATGAACA ATAAAACTGT
2581 CTGCTTACAT AAACAGTAAT ACAAGGGGTG TTATGAGCCA TATTCAACGG GAAACGTCGA
2641 GGCCGCGATT AAATTCCAAC ATGGATGCTG ATTTATATGG GTATAAATGG GCTCGCGATA
2701 ATGTCGGGCA ATCAGGTGCG ACAATCTATC GCTTGTATGG GAAGCCCGAT GCGCCAGAGT
2761 TGTTTCTGAA ACATGGCAAA GGTAGCGTTG CCAATGATGT TACAGATGAG ATGGTCAGAC
2821 TAAACTGGCT GACGGAATTT ATGCCTCTTC CGACCATCAA GCATTTTATC CGTACTCCTG
2881 ATGATGCATG GTTACTCACC ACTGCGATCC CCGGAAAAAC AGCATTCCAG GTATTAGAAG
2941 AATATCCTGA TTCAGGTGAA AATATTGTTG ATGCGCTGGC AGTGTTCCTG CGCCGGTTGC
3001 ATTCGATTCC TGTTTGTAAT TGTCCTTTTA ACAGCGATCG CGTATTTCGT CTCGCTCAGG
3061 CGCAATCACG AATGAATAAC GGTTTGGTTG ATGCGAGTGA TTTTGATGAC GAGCGTAATG
3121 GCTGGCCTGT TGAACAAGTC TGGAAAGAAA TGCATAAACT TTTGCCATTC TCACCGGATT
3181 CAGTCGTCAC TCATGGTGAT TTCTCACTTG ATAACCTTAT TTTTGACGAG GGGAAATTAA
3241 TAGGTTGTAT TGATGTTGGA CGAGTCGGAA TCGCAGACCG ATACCAGGAT CTTGCCATCC
3301 TATGGAACTG CCTCGGTGAG TTTTCTCCTT CATTACAGAA ACGGCTTTTT CAAAAATATG
3361 GTATTGATAA TCCTGATATG AATAAATTGC AGTTTCATTT GATGCTCGAT GAGTTTTTCT
3421 AATCAGAATT GGTTAATTGG TTGTAACACT GGCAGAGCAT TACGCTGACT TGACGGGACG
3481 GCGCAAGCTC ATGACCAAAA TCCCTTAACG TGAGTTACGC GTCGTTCCAC TGAGCGTCAG
3541 ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT
3601 GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC
3661 CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC
3721 TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG
3781 CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT
3841 TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT
3901 GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC
3961 ATTGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA
4021 GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA
4081 GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG
4141 GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT
4201 GGCCTTTTGC TCACATGTT
//