From Tol2Kit
LOCUS 228.p5E-MCS 2810 bp ds-DNA circular 18-DEC-2007
REFERENCE 1
AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
Parant JM, Yost HJ, Kanki JP, Chien CB.
TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2
transposon transgenesis constructs.
JOURNAL Developmental Dynamics 236(11):3088-99 (2007).
PUBMED 17937395
COMMENT 228.p5E-MCS, Tol2kit v1.0
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_recomb 668..688
/label=attB4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_recomb 593..688
/label=attL4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_recomb 856..1012
/label=attR1
/ApEinfo_fwdcolor=#3fd0ff
/ApEinfo_revcolor=#3fd0ff
misc_feature 860..873
/label=att1_shared
/ApEinfo_fwdcolor=#32a6cc
/ApEinfo_revcolor=#32a6cc
misc_feature 668..688
/label=att4_shared
/ApEinfo_fwdcolor=#4a33cc
/ApEinfo_revcolor=#4a33cc
misc_feature 689..855
/label=pBS MCS T7-T3
/ApEinfo_fwdcolor=#dddddd
/ApEinfo_revcolor=#dddddd
rep_origin 2134..2807
/label=pUC ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
CDS 1204..2013
/label=kanR
/ApEinfo_fwdcolor=#ff7f3f
/ApEinfo_revcolor=#ff7f3f
primer_bind complement(1071..1091)
/label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind 537..552
/label=M13F
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(836..855)
/label=T3
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(1048..1067)
/label=T7
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind 689..711
/label=T7 primer
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
terminator 268..295
/label=T2
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
terminator 427..470
/label=T1
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
ORIGIN
1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
541 AACGACGGCC AGTCTTAAGC TCGGGCCCGC GTTAACGCTA CCATGGAGCT CCAAATAATG
601 ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA ATGCTTTTTT
661 ATAATGCCAA CTTTGTATAG AAAAGTTGGT AATACGACTC ACTATAGGGC GAATTGGGTA
721 CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC CTGCAGCCCG
781 GGGGATCCAC TAGTTCTAGA GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT TTGTTCCCTT
841 TAGTGAGGGT TAATTCAAGT TTGTACAAAA AAGTTGAACG AGAAACGTAA AATGATATAA
901 ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC
961 AACATATGCA GTCACTATGA ACCAACTACT TAGATGGTAT TAGTGACCTG TAGAATTCGA
1021 GCTCTAGAGC TGCAGGGCGG CCGCGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA
1081 GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT
1141 AAAAATATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT
1201 GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT
1261 GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT
1321 CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT
1381 GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT
1441 CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC
1501 CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT
1561 GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT
1621 AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT
1681 GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA
1741 ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT
1801 GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA
1861 ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT
1921 TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG
1981 CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC
2041 TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC
2101 GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT
2161 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA
2221 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC
2281 AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC
2341 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT
2401 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG
2461 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC
2521 TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG
2581 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG
2641 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT
2701 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC
2761 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT
//