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LOCUS       380.p5E-h2afx        3604 bp ds-DNA     circular     18-DEC-2007
REFERENCE   1
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, 
			Parant JM, Yost HJ, Kanki JP, Chien CB.
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2 
			transposon transgenesis constructs.
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
  PUBMED    17937395
COMMENT     380.p5E-h2afx, Tol2kit v1.0
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     668..688
                     /label=attB4
                     /ApEinfo_fwdcolor=#5d40ff
                     /ApEinfo_revcolor=#5d40ff
     misc_recomb     593..688
                     /label=attL4
                     /ApEinfo_fwdcolor=#5d40ff
                     /ApEinfo_revcolor=#5d40ff
     misc_recomb     1650..1806
                     /label=attR1
                     /ApEinfo_fwdcolor=#3fd0ff
                     /ApEinfo_revcolor=#3fd0ff
     misc_feature    1654..1667
                     /label=att1_shared
                     /ApEinfo_fwdcolor=#32a6cc
                     /ApEinfo_revcolor=#32a6cc
     misc_feature    668..688
                     /label=att4_shared
                     /ApEinfo_fwdcolor=#4a33cc
                     /ApEinfo_revcolor=#4a33cc
     rep_origin      2928..3601
                     /label=pUC ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     CDS             1998..2807
                     /label=kanR
                     /ApEinfo_fwdcolor=#ff7f3f
                     /ApEinfo_revcolor=#ff7f3f
     primer_bind     complement(1865..1885)
                     /label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     537..552
                     /label=M13F
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        complement(1842..1861)
                     /label=T7
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     terminator      268..295
                     /label=T2
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
     terminator      427..470
                     /label=T1
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
     misc_feature    691..1619
                     /label=h2afx genomic, 1-929 bp upstream of tx start
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    1620..1647
                     /label=partial h2afx 5' UTR
                     /ApEinfo_fwdcolor=#35c0c7
                     /ApEinfo_revcolor=#35c0c7
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCGC GTTAACGCTA CCATGGAGCT CCAAATAATG
      601 ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA ATGCTTTTTT
      661 ATAATGCCAA CTTTGTATAG AAAAGTTGGG AAAATAGTCT GCTGGGGTTC CCCACCCATA
      721 CGACTCAATG CAATTCTCAG ACTTTTGTCT TTCATTCCAA ATAACCTGAA CTTATAATAA
      781 AGGCAAGGGT GTTCTAACCA AAATAATAGC TACAGGAAAG CATTTTAGCA ATTATACAGT
      841 GAATTGCAGG TCACGAGTCA ATGGCTCAGC GCTTTATAGT CATTTTGGAG CATAAAGCCT
      901 GAAAAATGTA TGAGCAAAAT GTTGTGATGA CAGCGAACAA TCTGAAGTGA AGGTGCTGCT
      961 GCTGGGCCTT CTGAGGTATT CGGAGCTTTA TATTGTTGTT CATTTGATGT TTAATTTATT
     1021 CTGAATAAAT TATTTTTGAA AAACCATTCC TCCTTTCTTA TTTCCCATTA TTAATCAGCT
     1081 AAAAGTGAAT TTCTCTAAAA TGCACACTGA ATCACTTTTA TTGTCACATC ACGACAAAGT
     1141 GATTTAATTC ATTGTGCATT CTAAAGAAAT CGCATACAAA TCACATTTTA AAGACATTTA
     1201 CATTGAGTGA ATTAAATCTA TAAAATATTA CGTCACAATA AGTGTACATG AGAGGGTTAA
     1261 ACAAATGAAA AAATTGGTTT TGCGTCAAAC TGTGTTAAAA TTCAGAGGGA ATATGTTCAA
     1321 TTGGTTTGAA CTTACCGCCG AAATGTGATT GAGGTAAAGA AAGACCAATA CTAGCGCAGA
     1381 CGAACCAAGC GAATCAATCA GCTCTCGCAT ATTCAGAAGC ACTCTGATTG GTTCGTTACT
     1441 TTGCGCTGTT TATGCACTGC AAGATCAAGC CAATGGGGGA TTACATCCGC TCTGGTTTCA
     1501 CCACCGACAA CCAATCAGGT CCGTACTTCC CTTCGCGCAA CCAATCAAAT GTGTGGGCGG
     1561 GACTATGCTT TGAACGTTCA ACCAGTCTTT AAATACTGCA GCAGTTTCAT TGCCAATGTA
     1621 CTGATTTCTT GCACACGCGA AGAGTTAGCC AAGTTTGTAC AAAAAAGTTG AACGAGAAAC
     1681 GTAAAATGAT ATAAATATCA ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA
     1741 ATACTGTAAA ACACAACATA TGCAGTCACT ATGAATCAAC TACTTAGATG GTATTAGTGA
     1801 CCTGTAGAAT TCGAGCTCTA GAGCTGCAGG GCGGCCGCGA TATCCCCTAT AGTGAGTCGT
     1861 ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT
     1921 ACATTGCACA AGATAAAAAT ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA
     1981 GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT
     2041 CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG
     2101 GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG
     2161 GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG
     2221 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC
     2281 TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG
     2341 GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT
     2401 GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA
     2461 ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC
     2521 AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG
     2581 GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG
     2641 TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG
     2701 GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG
     2761 ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA
     2821 ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC
     2881 CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
     2941 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
     3001 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
     3061 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT
     3121 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
     3181 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
     3241 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
     3301 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
     3361 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
     3421 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
     3481 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
     3541 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
     3601 TGTT     
//