From Tol2Kit
LOCUS 380.p5E-h2afx 3604 bp ds-DNA circular 18-DEC-2007
REFERENCE 1
AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
Parant JM, Yost HJ, Kanki JP, Chien CB.
TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2
transposon transgenesis constructs.
JOURNAL Developmental Dynamics 236(11):3088-99 (2007).
PUBMED 17937395
COMMENT 380.p5E-h2afx, Tol2kit v1.0
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_recomb 668..688
/label=attB4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_recomb 593..688
/label=attL4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_recomb 1650..1806
/label=attR1
/ApEinfo_fwdcolor=#3fd0ff
/ApEinfo_revcolor=#3fd0ff
misc_feature 1654..1667
/label=att1_shared
/ApEinfo_fwdcolor=#32a6cc
/ApEinfo_revcolor=#32a6cc
misc_feature 668..688
/label=att4_shared
/ApEinfo_fwdcolor=#4a33cc
/ApEinfo_revcolor=#4a33cc
rep_origin 2928..3601
/label=pUC ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
CDS 1998..2807
/label=kanR
/ApEinfo_fwdcolor=#ff7f3f
/ApEinfo_revcolor=#ff7f3f
primer_bind complement(1865..1885)
/label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind 537..552
/label=M13F
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(1842..1861)
/label=T7
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
terminator 268..295
/label=T2
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
terminator 427..470
/label=T1
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
misc_feature 691..1619
/label=h2afx genomic, 1-929 bp upstream of tx start
/ApEinfo_fwdcolor=cyan
/ApEinfo_revcolor=green
misc_feature 1620..1647
/label=partial h2afx 5' UTR
/ApEinfo_fwdcolor=#35c0c7
/ApEinfo_revcolor=#35c0c7
ORIGIN
1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
541 AACGACGGCC AGTCTTAAGC TCGGGCCCGC GTTAACGCTA CCATGGAGCT CCAAATAATG
601 ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA ATGCTTTTTT
661 ATAATGCCAA CTTTGTATAG AAAAGTTGGG AAAATAGTCT GCTGGGGTTC CCCACCCATA
721 CGACTCAATG CAATTCTCAG ACTTTTGTCT TTCATTCCAA ATAACCTGAA CTTATAATAA
781 AGGCAAGGGT GTTCTAACCA AAATAATAGC TACAGGAAAG CATTTTAGCA ATTATACAGT
841 GAATTGCAGG TCACGAGTCA ATGGCTCAGC GCTTTATAGT CATTTTGGAG CATAAAGCCT
901 GAAAAATGTA TGAGCAAAAT GTTGTGATGA CAGCGAACAA TCTGAAGTGA AGGTGCTGCT
961 GCTGGGCCTT CTGAGGTATT CGGAGCTTTA TATTGTTGTT CATTTGATGT TTAATTTATT
1021 CTGAATAAAT TATTTTTGAA AAACCATTCC TCCTTTCTTA TTTCCCATTA TTAATCAGCT
1081 AAAAGTGAAT TTCTCTAAAA TGCACACTGA ATCACTTTTA TTGTCACATC ACGACAAAGT
1141 GATTTAATTC ATTGTGCATT CTAAAGAAAT CGCATACAAA TCACATTTTA AAGACATTTA
1201 CATTGAGTGA ATTAAATCTA TAAAATATTA CGTCACAATA AGTGTACATG AGAGGGTTAA
1261 ACAAATGAAA AAATTGGTTT TGCGTCAAAC TGTGTTAAAA TTCAGAGGGA ATATGTTCAA
1321 TTGGTTTGAA CTTACCGCCG AAATGTGATT GAGGTAAAGA AAGACCAATA CTAGCGCAGA
1381 CGAACCAAGC GAATCAATCA GCTCTCGCAT ATTCAGAAGC ACTCTGATTG GTTCGTTACT
1441 TTGCGCTGTT TATGCACTGC AAGATCAAGC CAATGGGGGA TTACATCCGC TCTGGTTTCA
1501 CCACCGACAA CCAATCAGGT CCGTACTTCC CTTCGCGCAA CCAATCAAAT GTGTGGGCGG
1561 GACTATGCTT TGAACGTTCA ACCAGTCTTT AAATACTGCA GCAGTTTCAT TGCCAATGTA
1621 CTGATTTCTT GCACACGCGA AGAGTTAGCC AAGTTTGTAC AAAAAAGTTG AACGAGAAAC
1681 GTAAAATGAT ATAAATATCA ATATATTAAA TTAGATTTTG CATAAAAAAC AGACTACATA
1741 ATACTGTAAA ACACAACATA TGCAGTCACT ATGAATCAAC TACTTAGATG GTATTAGTGA
1801 CCTGTAGAAT TCGAGCTCTA GAGCTGCAGG GCGGCCGCGA TATCCCCTAT AGTGAGTCGT
1861 ATTACATGGT CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT
1921 ACATTGCACA AGATAAAAAT ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA
1981 GTAATACAAG GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT
2041 CCAACATGGA TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG
2101 GTGCGACAAT CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG
2161 GCAAAGGTAG CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG
2221 AATTTATGCC TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC
2281 TCACCACTGC GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG
2341 GTGAAAATAT TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT
2401 GTAATTGTCC TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA
2461 ATAACGGTTT GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC
2521 AAGTCTGGAA AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG
2581 GTGATTTCTC ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG
2641 TTGGACGAGT CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG
2701 GTGAGTTTTC TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG
2761 ATATGAATAA ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA
2821 ATTGGTTGTA ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC
2881 CAAAATCCCT TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA
2941 TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA
3001 AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA
3061 AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT
3121 TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT
3181 TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT
3241 AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT
3301 TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA
3361 CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG
3421 AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC
3481 GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA
3541 AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA
3601 TGTT
//