From Tol2Kit
LOCUS 394.pDestTol2pA2 5883 bp ds-DNA circular 18-DEC-2007
REFERENCE 1
AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
Parant JM, Yost HJ, Kanki JP, Chien CB.
TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2
transposon transgenesis constructs.
JOURNAL Developmental Dynamics 236(11):3088-99 (2007).
PUBMED 17937395
COMMENT 394.pDestTol2pA2, Tol2kit v1.0
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_recomb 2676..2800
/label=attR3
/ApEinfo_fwdcolor=#5370ff
/ApEinfo_revcolor=#5370ff
misc_recomb 1097..1221
/label=attR4
/ApEinfo_fwdcolor=#5d40ff
/ApEinfo_revcolor=#5d40ff
misc_feature 2780..2794
/label=att3_shared
/ApEinfo_fwdcolor=#4259cc
/ApEinfo_revcolor=#4259cc
misc_feature 1097..1117
/label=att4_shared
/ApEinfo_fwdcolor=#4a33cc
/ApEinfo_revcolor=#4a33cc
polyA_signal 2840..3037
/label=SV40 late polyA (from pCS2+)
/ApEinfo_fwdcolor=#ffff7f
/ApEinfo_revcolor=#ffff7f
rep_origin complement(4081..4754)
/label=pUC ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
rep_origin complement(4122..4804)
/label=colE1 ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
CDS complement(4902..5759)
/label=ampR
/ApEinfo_fwdcolor=#ff7f00
/ApEinfo_revcolor=#ff7f00
CDS complement(1908..2567)
/label=cmR
/ApEinfo_fwdcolor=#ffbf00
/ApEinfo_revcolor=#ffbf00
misc_feature complement(1261..1566)
/label=ccdB
/ApEinfo_fwdcolor=#ffbf3f
/ApEinfo_revcolor=#ffbf3f
primer_bind 1061..1082
/label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind complement(3733..3754)
/label=M13R(1)
/ApEinfo_label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind complement(2809..2824)
/label=M13F
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(3698..3717)
/label=T3
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
LTR complement(514..530)
/label=Tol2 3' TIR
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
LTR complement(3594..3612)
/label=Tol2 5' TIR
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
misc_structure complement(3096..3430)
/label=Tol2 exon1 bp1-335 of 461
/ApEinfo_fwdcolor=#aa0000
/ApEinfo_revcolor=#aa0000
misc_structure complement(703..1049)
/label=Tol2 exon4 136-482
/ApEinfo_fwdcolor=#aa0000
/ApEinfo_revcolor=#aa0000
misc_structure complement(3613..3649)
/label=medaka tyrosinase ex5 frag
/ApEinfo_fwdcolor=#006600
/ApEinfo_revcolor=#006600
gene 228..514
/label=medaka tyrosinase genomic (from AB010101)
/ApEinfo_fwdcolor=#006600
/ApEinfo_revcolor=#006600
ORIGIN
1 CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA
61 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA
121 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG
181 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACGC TGGCTAAGAA
241 CTCATCAGCC TCCCCGGTCC ATCTACCCAC GTACCAATGC ACCAATTGGC CACAATGACG
301 GCTACTACAT GGTGCCATTC CTTCCTCTTT ATAGGAATGG AGACTACCTC CTGTCCAACA
361 AGGCTCTTGG ATACGAGTAC GCCTACCTGT TGGACCCAGG TCATTGCACA ACACCAGAAA
421 TGCCCTCTGA TCTGCAAAAG ACGTGAATAT CTGTTCAGAC ACCCATATCC ACTCTGTTCC
481 ACACAGGTCA GAGGTTTGTC CAGGAGTTCT TGACAGAGGT GTAAAAAGTA CTCAAAAATT
541 TTACTCAAGT GAAAGTACAA GTACTTAGGG AAAATTTTAC TCAATTAAAA GTAAAAGTAT
601 CTGGCTAGAA TCTTACTTGA GTAAAAGTAA AAAAGTACTC CATTAAAATT GTACTTGAGT
661 ATTAAGGAAG TAAAAGTAAA AGCAAGAAAG AAAACTAGAG ATTCTTGTTT AAGCTTTTAA
721 TCTCAAAAAA CATTAAATGA AATGCATACA AGGTTTTATC CTGCTTTAGA ACTGTTTGTA
781 TTTAATTATC AAACTATAAG ACAGACAATC TAATGCCAGT ACACGCTACT CAAAGTTGTA
841 AAACCTCAGA TTTAACTTCA GTAGAAGCTG ATTCTCAAAA TTGTTAGTGT CAAGCCTAGC
901 TCTTTTGGGG CTGAAAAGCA ATCCTGCAGT GCTGAAAAGC CTCTCACAGG CAGCCGATGC
961 GGGAAGAGGT GTATTAGTCT TGATAGAGAG GCTGCAAATA GCAGGAAACG TGAGCAGAGA
1021 CTCCCTGGTG TCTGAAACAC AGGCCAGATG GGCCCTCGAG CAGGAAACAG CTATGACCAT
1081 GATTACGCCA AGCTATCAAC TTTGTATAGA AAAGTTGAAC GAGAAACGTA AAATGATATA
1141 AATATCAATA TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA
1201 CAACATATCC AGTCACTATG GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT
1261 TTATATTCCC CAGAACATCA GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG
1321 ATCAGCCACT TCTTCCCCGA TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT
1381 GCGCCAGCTT TCATCCCCGA TATGCACCAC CGGGTAAAGT TCACGGGGGA CTTTATCTGA
1441 CAGCAGACGT GCACTGGCCA GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC
1501 ACTCTGTACA TCCACAAACA GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA
1561 CTGCATTTCA CCAGCCCCTG TTCTCGTCGG CAAAGGAGCC GTTCATTTCA ATAAACCGGG
1621 CGACCTCAGC CATCCCTTCC TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG
1681 CTTCATTCTG CATGGTTGTG CTTACCGAAC CGGAGATATT GACATCATAT ATGCCTTGAG
1741 CAACTGATAG CTGTCGCTGT CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT
1801 TTTGACATAC TTCGGGTATA CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC
1861 AAATACGCAT ACTGTTATCT GGCTTTTAGT AAGCCGGATC CTCTAGATTA CGCCCCGCCC
1921 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA
1981 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA
2041 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA
2101 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT
2161 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA
2221 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA
2281 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT
2341 GCCATACGGA ATTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA
2401 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG
2461 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC
2521 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATTTT AGCTTCCTTA
2581 GCTCCTGAAA ATCTCGACGG ATCCTAACTC AAAATCCACA CATTATACGA GCCGGAAGCA
2641 TAAAGTGTAA AGCCTGGGGG TGCCTAATGC GGCCGCCATA GTGACTGGAT ATGTTGTGTT
2701 TTACAGTATT ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA
2761 TCATTTTACG TTTCTCGTTC AACTTTATTA TACATAGTTG ATAATTCACT GGCCGTCGTT
2821 TTACGGTACC ATCGATGATG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC
2881 CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT
2941 ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT
3001 GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG
3061 TGGTATGGCT GATTATGATC CTCTAGATCA GATCTGCGAA GATACGGCCA CGGGTGCTCT
3121 TGATCCTGTG GCTGATTTTG GACTGTGCTG CTCGCAGCTG CTGATGAATC ACATACTTCC
3181 TCCATTTTCT TCCACTGATT GACTGTTATA ATTTCCCTAA TTTCCAGGTC AAGGTGCTGT
3241 GCATTGTGGT AATAGATGTG ACATGACGTC ACTTCCAAAG GACCAATGAA CATGTCTGAC
3301 CAATTTCATA TAATGTGAAA ACGATTTTCA TAGGCAGAAT AAATAACATT TAAATTAAAC
3361 TGGGCATCAG CGCAATTCAA TTGGTTTGGT AATAGCAAGG GAAAATAGAA TGAAGTGATC
3421 TCCAAAAAAT AAGTACTTTT TGACTGTAAA TAAAATTGTA AGGAGTAAAA AGTACTTTTT
3481 TTTCTAAAAA AATGTAATTA AGTAAAAGTA AAAGTATTGA TTTTTAATTG TACTCAAGTA
3541 AAGTAAAAAT CCCCAAAAAT AATACTTAAG TACAGTAATC AAGTAAAATT ACTCAAGTAC
3601 TTTACACCTC TGGTTCTTGA CCCCCTACCT TCAGCAAGCC CAGCAGATCC ACTAGTTCTA
3661 GAGCGGCCGC CACCGCGGTG GAGCTCCAGC TTTTGTTCCC TTTAGTGAGG GTTAATTGCG
3721 CGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT
3781 CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC
3841 TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC
3901 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT
3961 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA
4021 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC
4081 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
4141 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
4201 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
4261 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
4321 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
4381 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
4441 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
4501 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
4561 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
4621 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
4681 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
4741 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
4801 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
4861 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
4921 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
4981 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
5041 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
5101 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
5161 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
5221 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
5281 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
5341 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
5401 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
5461 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
5521 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
5581 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
5641 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
5701 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
5761 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
5821 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
5881 GTG
//