From Tol2Kit
LOCUS 384.pME-EGFPCAAX 3345 bp ds-DNA circular 18-DEC-2007
REFERENCE 1
AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS,
Parant JM, Yost HJ, Kanki JP, Chien CB.
TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2
transposon transgenesis constructs.
JOURNAL Developmental Dynamics 236(11):3088-99 (2007).
PUBMED 17937395
COMMENT 384.pME-EGFPCAAX, Tol2kit v1.0
COMMENT ApEinfo:methylated:1
FEATURES Location/Qualifiers
misc_recomb 569..668
/label=attL1
/ApEinfo_fwdcolor=#3fd0ff
/ApEinfo_revcolor=#3fd0ff
misc_recomb 1470..1569
/label=attL2
/ApEinfo_fwdcolor=#49a0ff
/ApEinfo_revcolor=#49a0ff
misc_feature 649..662
/label=att1_shared
/ApEinfo_fwdcolor=#32a6cc
/ApEinfo_revcolor=#32a6cc
misc_feature 1476..1491
/label=att2_shared
/ApEinfo_fwdcolor=#3a80cc
/ApEinfo_revcolor=#3a80cc
misc_structure 1406..1468
/label=CAAX box from Harvey ras
/ApEinfo_fwdcolor=#ff0000
/ApEinfo_revcolor=#ff0000
CDS 674..1390
/label=EGFP
/ApEinfo_fwdcolor=#00ff00
/ApEinfo_revcolor=#00ff00
misc_structure 865..878
/label=EGFP-like chromophore
/ApEinfo_fwdcolor=#00cc00
/ApEinfo_revcolor=#00cc00
rep_origin 2669..3342
/label=pUC ori
/ApEinfo_fwdcolor=#999999
/ApEinfo_revcolor=#999999
CDS 1739..2548
/label=kanR
/ApEinfo_fwdcolor=#ff7f3f
/ApEinfo_revcolor=#ff7f3f
primer_bind complement(1606..1626)
/label=M13R
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
primer_bind 537..552
/label=M13F
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
promoter complement(1583..1602)
/label=T7
/ApEinfo_fwdcolor=#777777
/ApEinfo_revcolor=#777777
terminator 268..295
/label=T2
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
terminator 427..470
/label=T1
/ApEinfo_fwdcolor=#bbbbbb
/ApEinfo_revcolor=#bbbbbb
ORIGIN
1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC
601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA
661 AGCAGGCTGG ACCATGGTGA GCAAGGGCGA GGAGCTGTTC ACCGGGGTGG TGCCCATCCT
721 GGTCGAGCTG GACGGCGACG TAAACGGCCA CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG
781 CGATGCCACC TACGGCAAGC TGACCCTGAA GTTCATCTGC ACCACCGGCA AGCTGCCCGT
841 GCCCTGGCCC ACCCTCGTGA CCACCCTGAC CTACGGCGTG CAGTGCTTCA GCCGCTACCC
901 CGACCACATG AAGCAGCACG ACTTCTTCAA GTCCGCCATG CCCGAAGGCT ACGTCCAGGA
961 GCGCACCATC TTCTTCAAGG ACGACGGCAA CTACAAGACC CGCGCCGAGG TGAAGTTCGA
1021 GGGCGACACC CTGGTGAACC GCATCGAGCT GAAGGGCATC GACTTCAAGG AGGACGGCAA
1081 CATCCTGGGG CACAAGCTGG AGTACAACTA CAACAGCCAC AACGTCTATA TCATGGCCGA
1141 CAAGCAGAAG AACGGCATCA AGGTGAACTT CAAGATCCGC CACAACATCG AGGACGGCAG
1201 CGTGCAGCTC GCCGACCACT ACCAGCAGAA CACCCCCATC GGCGACGGCC CCGTGCTGCT
1261 GCCCGACAAC CACTACCTGA GCACCCAGTC CGCCCTGAGC AAAGACCCCA ACGAGAAGCG
1321 CGATCACATG GTCCTGCTGG AGTTCGTGAC CGCCGCCGGG ATCACTCTCG GCATGGACGA
1381 GCTGTACAAG GGAGGAGGAA GATCTAAGCT GAACCCTCCT GATGAGAGTG GCCCCGGCTG
1441 CATGAGCTGC AAGTGTGTGC TCTCCTGAGA CCCAGCTTTC TTGTACAAAG TTGGCATTAT
1501 AAGAAAGCAT TGCTTATCAA TTTGTTGCAA CGAACAGGTC ACTATCAGTC AAAATAAAAT
1561 CATTATTTGC CATCCAGCTG ATATCCCCTA TAGTGAGTCG TATTACATGG TCATAGCTGT
1621 TTCCTGGCAG CTCTGGCCCG TGTCTCAAAA TCTCTGATGT TACATTGCAC AAGATAAAAT
1681 AATATCATCA TGAACAATAA AACTGTCTGC TTACATAAAC AGTAATACAA GGGGTGTTAT
1741 GAGCCATATT CAACGGGAAA CGTCGAGGCC GCGATTAAAT TCCAACATGG ATGCTGATTT
1801 ATATGGGTAT AAATGGGCTC GCGATAATGT CGGGCAATCA GGTGCGACAA TCTATCGCTT
1861 GTATGGGAAG CCCGATGCGC CAGAGTTGTT TCTGAAACAT GGCAAAGGTA GCGTTGCCAA
1921 TGATGTTACA GATGAGATGG TCAGACTAAA CTGGCTGACG GAATTTATGC CTCTTCCGAC
1981 CATCAAGCAT TTTATCCGTA CTCCTGATGA TGCATGGTTA CTCACCACTG CGATCCCCGG
2041 AAAAACAGCA TTCCAGGTAT TAGAAGAATA TCCTGATTCA GGTGAAAATA TTGTTGATGC
2101 GCTGGCAGTG TTCCTGCGCC GGTTGCATTC GATTCCTGTT TGTAATTGTC CTTTTAACAG
2161 CGATCGCGTA TTTCGTCTCG CTCAGGCGCA ATCACGAATG AATAACGGTT TGGTTGATGC
2221 GAGTGATTTT GATGACGAGC GTAATGGCTG GCCTGTTGAA CAAGTCTGGA AAGAAATGCA
2281 TAAACTTTTG CCATTCTCAC CGGATTCAGT CGTCACTCAT GGTGATTTCT CACTTGATAA
2341 CCTTATTTTT GACGAGGGGA AATTAATAGG TTGTATTGAT GTTGGACGAG TCGGAATCGC
2401 AGACCGATAC CAGGATCTTG CCATCCTATG GAACTGCCTC GGTGAGTTTT CTCCTTCATT
2461 ACAGAAACGG CTTTTTCAAA AATATGGTAT TGATAATCCT GATATGAATA AATTGCAGTT
2521 TCATTTGATG CTCGATGAGT TTTTCTAATC AGAATTGGTT AATTGGTTGT AACACTGGCA
2581 GAGCATTACG CTGACTTGAC GGGACGGCGC AAGCTCATGA CCAAAATCCC TTAACGTGAG
2641 TTACGCGTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA
2701 TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT
2761 GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG
2821 AGCGCAGATA CCAAATACTG TTCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA
2881 CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG
2941 TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA
3001 GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC
3061 CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA
3121 GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC
3181 AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG
3241 TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC
3301 CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTT
//