From Tol2Kit
LOCUS 234.pME-H2A-mCherry 3651 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, Yost HJ, Kanki JP, Chien CB. TITLE The Tol2kit: a multisite gateway-based construction kit for Tol2 transposon transgenesis constructs. JOURNAL Developmental Dynamics 236(11):3088-99 (2007). PUBMED 17937395 COMMENT 234.pME-H2A-mCherry, Tol2kit v1.0 COMMENT ApEinfo:methylated:1 FEATURES Location/Qualifiers misc_recomb 569..668 /label=attL1 /ApEinfo_fwdcolor=#3fd0ff /ApEinfo_revcolor=#3fd0ff misc_recomb 1776..1875 /label=attL2 /ApEinfo_fwdcolor=#49a0ff /ApEinfo_revcolor=#49a0ff misc_feature 649..662 /label=att1_shared /ApEinfo_fwdcolor=#32a6cc /ApEinfo_revcolor=#32a6cc misc_feature 1782..1797 /label=att2_shared /ApEinfo_fwdcolor=#3a80cc /ApEinfo_revcolor=#3a80cc CDS 674..1057 /label=zf H2A.F/Z /ApEinfo_fwdcolor=#ff0000 /ApEinfo_revcolor=#ff0000 CDS 1064..1771 /label=mCherry (Svoboda) /ApEinfo_fwdcolor=#dc143c /ApEinfo_revcolor=#dc143c rep_origin 2975..3648 /label=pUC ori /ApEinfo_fwdcolor=#999999 /ApEinfo_revcolor=#999999 CDS 2045..2854 /label=kanR /ApEinfo_fwdcolor=#ff7f3f /ApEinfo_revcolor=#ff7f3f primer_bind complement(1912..1932) /label=M13R /ApEinfo_fwdcolor=#777777 /ApEinfo_revcolor=#777777 primer_bind 537..552 /label=M13F /ApEinfo_fwdcolor=#777777 /ApEinfo_revcolor=#777777 promoter complement(1889..1908) /label=T7 /ApEinfo_fwdcolor=#777777 /ApEinfo_revcolor=#777777 terminator 268..295 /label=T2 /ApEinfo_fwdcolor=#bbbbbb /ApEinfo_revcolor=#bbbbbb terminator 427..470 /label=T1 /ApEinfo_fwdcolor=#bbbbbb /ApEinfo_revcolor=#bbbbbb ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTAC ACCATGGCAG GTGGAAAAGC AGGTAAAGAC AGTGGCAAAG CCAAGGCGAA 721 AGCAGTGTCT CGCTCGCAAA GAGCTGGTCT TCAGTTTCCA GTGGGACGAA TCCACAGGCA 781 CTTGAAGACA CGCACTACAA GCCATGGTCG AGTAGGAGCA ACAGCAGCCG TTTACAGCGC 841 AGCCATTCTT GAATATCTCA CAGCTGAAGT TTTGGAGTTG GCGGGAAATG CTTCAAAAGA 901 CCTGAAGGTG AAGCGCATCA CTCCTCGACA TTTACAGCTG GCCATTCGAG GAGATGAGGA 961 GCTCGATTCC CTTATCAAGG CCACTATTGC TGGAGGAGGT GTGATTCCAC ACATCCACAA 1021 GTCTCTGATT GGTAAGAAGG GCCAGCAGAA AACCGCAGGG GGGATGGTGA GCAAGGGCGA 1081 GGAGGACAAC ATGGCCATCA TCAAGGAGTT CATGCGCTTC AAGGTGCACA TGGAGGGCTC 1141 CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG GGCCGCCCCT ACGAGGGCAC 1201 CCAGACCGCC AAGCTGAAGG TGACCAAGGG CGGCCCCCTG CCCTTCGCCT GGGACATCCT 1261 GTCCCCTCAG TTCATGTACG GCTCCAAGGC CTACGTGAAG CACCCCGCCG ACATCCCCGA 1321 CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG CGCGTGATGA ACTTCGAGGA 1381 CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG GACGGCGAGT TCATCTACAA 1441 GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC GTAATGCAGA AGAAGACCAT 1501 GGGCTGGGAG GCCTCCTCCG AGCGGATGTA CCCCGAGGAC GGCGCCCTGA AGGGCGAGAT 1561 CAAGCAGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC GCCGAGGTCA AGACCACCTA 1621 CAAGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAC GTCAACATCA AGCTGGACAT 1681 CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC GAGCGCGCCG AGGGCCGCCA 1741 CTCCACCGGC GGCATGGACG AGCTGTACAA GTAAGACCCA GCTTTCTTGT ACAAAGTTGG 1801 CATTATAAGA AAGCATTGCT TATCAATTTG TTGCAACGAA CAGGTCACTA TCAGTCAAAA 1861 TAAAATCATT ATTTGCCATC CAGCTGATAT CCCCTATAGT GAGTCGTATT ACATGGTCAT 1921 AGCTGTTTCC TGGCAGCTCT GGCCCGTGTC TCAAAATCTC TGATGTTACA TTGCACAAGA 1981 TAAAATAATA TCATCATGAA CAATAAAACT GTCTGCTTAC ATAAACAGTA ATACAAGGGG 2041 TGTTATGAGC CATATTCAAC GGGAAACGTC GAGGCCGCGA TTAAATTCCA ACATGGATGC 2101 TGATTTATAT GGGTATAAAT GGGCTCGCGA TAATGTCGGG CAATCAGGTG CGACAATCTA 2161 TCGCTTGTAT GGGAAGCCCG ATGCGCCAGA GTTGTTTCTG AAACATGGCA AAGGTAGCGT 2221 TGCCAATGAT GTTACAGATG AGATGGTCAG ACTAAACTGG CTGACGGAAT TTATGCCTCT 2281 TCCGACCATC AAGCATTTTA TCCGTACTCC TGATGATGCA TGGTTACTCA CCACTGCGAT 2341 CCCCGGAAAA ACAGCATTCC AGGTATTAGA AGAATATCCT GATTCAGGTG AAAATATTGT 2401 TGATGCGCTG GCAGTGTTCC TGCGCCGGTT GCATTCGATT CCTGTTTGTA ATTGTCCTTT 2461 TAACAGCGAT CGCGTATTTC GTCTCGCTCA GGCGCAATCA CGAATGAATA ACGGTTTGGT 2521 TGATGCGAGT GATTTTGATG ACGAGCGTAA TGGCTGGCCT GTTGAACAAG TCTGGAAAGA 2581 AATGCATAAA CTTTTGCCAT TCTCACCGGA TTCAGTCGTC ACTCATGGTG ATTTCTCACT 2641 TGATAACCTT ATTTTTGACG AGGGGAAATT AATAGGTTGT ATTGATGTTG GACGAGTCGG 2701 AATCGCAGAC CGATACCAGG ATCTTGCCAT CCTATGGAAC TGCCTCGGTG AGTTTTCTCC 2761 TTCATTACAG AAACGGCTTT TTCAAAAATA TGGTATTGAT AATCCTGATA TGAATAAATT 2821 GCAGTTTCAT TTGATGCTCG ATGAGTTTTT CTAATCAGAA TTGGTTAATT GGTTGTAACA 2881 CTGGCAGAGC ATTACGCTGA CTTGACGGGA CGGCGCAAGC TCATGACCAA AATCCCTTAA 2941 CGTGAGTTAC GCGTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC 3001 TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC 3061 AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT 3121 CAGCAGAGCG CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT 3181 CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC 3241 TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA 3301 GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC 3361 CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG 3421 GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA 3481 GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT 3541 TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA 3601 CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT T //