From Tol2Kit
(New page: === Construction details === p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2-MT, using primers to add att sequences, followed by a BP reaction....) |
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=== Construction details === | === Construction details === | ||
| − | p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2 | + | p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2+MT, using primers to add att sequences, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. |
=== Crudely annotated sequence === | === Crudely annotated sequence === | ||
Revision as of 03:02, 24 March 2007
Construction details
p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2+MT, using primers to add att sequences, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
p3E-MTpA sequence
Crude map
Screenshot from Sequencher showing locations of components:
