From Tol2Kit
(New page: === Construction details === pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pC...) |
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pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pCS2FA, a version of pCS2+ modified by adding FseI and AscI sites. Sequencing confirms that all bases of the insert are correct. | pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pCS2FA, a version of pCS2+ modified by adding FseI and AscI sites. Sequencing confirms that all bases of the insert are correct. | ||
| − | The original transposase clone included start and stop codons upstream of the transposase cds, and a polyA (AAAAA) rather than a polyadenylation ''signal''. | + | The original transposase clone included start and stop codons upstream of the transposase cds, and a polyA sequence (AAAAA) rather than a polyadenylation ''signal''. |
=== Crudely annotated sequence === | === Crudely annotated sequence === | ||
Revision as of 03:34, 24 March 2007
Construction details
pCS2FA-transposase was made by PCRing the Tol2 transpoase cds from the original Kawakami clone, adding restriction sites, then conventionally subcloned into pCS2FA, a version of pCS2+ modified by adding FseI and AscI sites. Sequencing confirms that all bases of the insert are correct.
The original transposase clone included start and stop codons upstream of the transposase cds, and a polyA sequence (AAAAA) rather than a polyadenylation signal.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pCS2FA-transposase sequence
Crude map
Screenshot from Sequencher showing locations of components:
