From Tol2Kit
m (→Construction details) |
|||
| Line 2: | Line 2: | ||
p3E-polyA was made by PCR amplification of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. | p3E-polyA was made by PCR amplification of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. | ||
| − | === | + | === Sequence === |
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[p3E-polyA Genbank]] | ||
| + | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[p3E-polyA sequence]] | [[p3E-polyA sequence]] | ||
Latest revision as of 03:51, 19 December 2007
Construction details
p3E-polyA was made by PCR amplification of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Sequence
Annotated sequence, Genbank format:
p3E-polyA Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
p3E-polyA sequence
Crude map
Screenshot from Sequencher showing locations of components:
