From Tol2Kit
(New page: === Construction details === p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2-MT, using primers to add att sequences, followed by a BP reaction....) |
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=== Construction details === | === Construction details === | ||
| − | p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2 | + | p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 late polyadenylation signal from pCS2+MT, using primers to add att sequences, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. |
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| + | === Sequence === | ||
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[p3E-MTpA Genbank]] | ||
| − | |||
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[p3E-MTpA sequence]] | [[p3E-MTpA sequence]] | ||
Latest revision as of 03:52, 19 December 2007
Construction details
p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 late polyadenylation signal from pCS2+MT, using primers to add att sequences, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Sequence
Annotated sequence, Genbank format:
p3E-MTpA Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
p3E-MTpA sequence
Crude map
Screenshot from Sequencher showing locations of components:
