From Tol2Kit
(New page: '''Construction details'''<br> pDestTol2pA2 was made from pDestTol2pA by cutting with AfeII/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part ...) |
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| − | + | === Construction details === | |
| − | pDestTol2pA2 was made from pDestTol2pA by cutting with | + | pDestTol2pA2 was made from pDestTol2pA by cutting with AfeI/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality. |
| + | |||
| + | === Sequence === | ||
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[pDestTol2pA2 Genbank]] | ||
| − | |||
FASTA file with the full-length sequence as well as sequences of individual components):<br> | FASTA file with the full-length sequence as well as sequences of individual components):<br> | ||
| − | [[ | + | [[pDestTol2pA2 sequence]] |
| + | |||
| + | Edited 7 June 2007 to add six bases (a KpnI site that was missing). | ||
| − | + | === Crude map === | |
Screenshot from Sequencher showing locations of components:<br> | Screenshot from Sequencher showing locations of components:<br> | ||
| + | [[Image:pDestTol2pA2.png]] | ||
Latest revision as of 03:55, 19 December 2007
Construction details
pDestTol2pA2 was made from pDestTol2pA by cutting with AfeI/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
Sequence
Annotated sequence, Genbank format:
pDestTol2pA2 Genbank
FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2pA2 sequence
Edited 7 June 2007 to add six bases (a KpnI site that was missing).
Crude map
Screenshot from Sequencher showing locations of components:
