From Tol2Kit
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pDestTol2CG2 was made from pDestTol2CG by cutting with AfeI/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality. | pDestTol2CG2 was made from pDestTol2CG by cutting with AfeI/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality. | ||
| − | === | + | === Sequence === |
| + | Annotated sequence, Genbank format:<br> | ||
| + | [[pDestTol2CG2 Genbank]] | ||
| + | |||
FASTA file with the full-length sequence as well as sequences of individual components):<br> | FASTA file with the full-length sequence as well as sequences of individual components):<br> | ||
[[pDestTol2CG2 sequence]] | [[pDestTol2CG2 sequence]] | ||
Latest revision as of 03:55, 19 December 2007
Construction details
pDestTol2CG2 was made from pDestTol2CG by cutting with AfeI/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
Sequence
Annotated sequence, Genbank format:
pDestTol2CG2 Genbank
FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2CG2 sequence
Edited 7 June 2007 to add six bases (a KpnI site that was missing).
Crude map
Screenshot from Sequencher showing locations of components:
