From Tol2Kit
m (P5E-hsp70 moved to P5E-hsp70l: Changing name to match official ZFIN gene name) |
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=== Construction details === | === Construction details === | ||
| − | p5E-'' | + | p5E-''hsp70l'' was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that ''hsp70l'' is the official ZFIN gene name (thanks to Ceri van Slyke of ZFIN for pointing this out); this is the same gene originally named ''hsp70'' by Halloran et al., 2000. |
| + | |||
Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock. | Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock. | ||
=== Sequence === | === Sequence === | ||
Annotated sequence, Genbank format:<br> | Annotated sequence, Genbank format:<br> | ||
| − | [[p5E- | + | [[p5E-hsp70l Genbank]] |
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
| − | [[p5E- | + | [[p5E-hsp70l sequence]] |
=== Crude map === | === Crude map === | ||
Screenshot from Sequencher showing locations of components:<br> | Screenshot from Sequencher showing locations of components:<br> | ||
[[Image:p5E-hsp70.png]] | [[Image:p5E-hsp70.png]] | ||
Revision as of 12:00, 1 April 2008
Construction details
p5E-hsp70l was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that hsp70l is the official ZFIN gene name (thanks to Ceri van Slyke of ZFIN for pointing this out); this is the same gene originally named hsp70 by Halloran et al., 2000.
Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.
Sequence
Annotated sequence, Genbank format:
p5E-hsp70l Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
p5E-hsp70l sequence
Crude map
Screenshot from Sequencher showing locations of components:
