Difference between revisions of "P5E-hsp70l Genbank"

Latest revision as of 12:01, 1 April 2008

LOCUS       222.p5E-hsp70        4163 bp ds-DNA     circular     09-DEC-2007
DEFINITION  
REFERENCE   1
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, 
			Parant JM, Yost HJ, Kanki JP, Chien CB.
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2 
			transposon transgenesis constructs.
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
  PUBMED    17937395
COMMENT     222.p5E-hsp70l, Tol2kit v1.0
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     593..688
                     /label=attL4
                     /ApEinfo_fwdcolor=#5d40ff
                     /ApEinfo_revcolor=#5d40ff
     misc_recomb     2209..2365
                     /label=attR1
                     /ApEinfo_fwdcolor=#3fd0ff
                     /ApEinfo_revcolor=#3fd0ff
     misc_feature    2213..2226
                     /label=att1_shared
                     /ApEinfo_fwdcolor=#32a6cc
                     /ApEinfo_revcolor=#32a6cc
     misc_feature    668..688
                     /label=att4_shared
                     /ApEinfo_fwdcolor=#4a33cc
                     /ApEinfo_revcolor=#4a33cc
     promoter        691..2207
                     /label=hsp70l promoter (AF158020)
                     /ApEinfo_fwdcolor=#ffffbf
                     /ApEinfo_revcolor=#ffffbf
     rep_origin      3487..4160
                     /label=pUC ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     CDS             2557..3366
                     /label=kanR
                     /ApEinfo_fwdcolor=#ff7f3f
                     /ApEinfo_revcolor=#ff7f3f
     primer_bind     complement(2424..2444)
                     /label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     537..552
                     /label=M13F
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        complement(2401..2420)
                     /label=T7
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     terminator      268..295
                     /label=T2
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
     terminator      427..470
                     /label=T1
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCGC GTTAACGCTA CCATGGAGCT CCAAATAATG
      601 ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA ATGCTTTTTT
      661 ATAATGCCAA CTTTGTATAG AAAAGTTGCT TCAGGGGTGT CGCTTGGTTA TTTCCAAAAA
      721 TCAAATTAAT TTTATTAAAC TATTAGAACG AGCATGTTTT GTCTATATGC TACAGAAGAT
      781 AAAAAATAAT AGGAGTTAAC AGTTATAAAA CAACACACTT TGTTTCTATT GATTGTTGAC
      841 CACACTGGGG TCTCATTAAG TTAGATTAAA GACACACTAA CTGGGTCAAA AGCAGCAGAT
      901 TGATTTCATA GCACCAGGGT AAACTTTCTA ACACTTTTAC GGCAATCATA TACATTAAAA
      961 TTAAATACAG ACCACGACTG AACAAGGAGG ATGATCTCCA ATATTAAACA AAGAGACTTG
     1021 TGCCTATTTC TCTGAGGGTA AACATGACCT CTCAAGTTAG CAAGTTGGTT TTAACACTAC
     1081 AAAAATAGTT AAGACTGCAA TCCCAGAATA AAGTATTGGT TTTAACCAAT CAATATAGTA
     1141 CAGTAAACAT CCATTTGTTT TGTTGAAACG TTAAACAAAT CTGACCAAAG CTATTAGCTT
     1201 ATATAAAACA GGTTTGCCTT CTATGTAGCT GAAAACACCA CAGGCCCGAT TTTGCTACTG
     1261 TGTAAAACAT TTCAGCAAGA TTTTTTTATT GCATTTTTTT TTACTGAATC GTTCAAACAT
     1321 TTTATCATTT TAGTTTGTTC ATTCATTGCA ACTGGAAAAA CAACACATCA CACAACCGCA
     1381 CATATTTCAG CAATAAGTAC AATAAAACAC TCAAATAAAA AAAACATTTT AAATCTCTTT
     1441 GTATTTTTGA CCGCTGTTTC GCGTAATTTC ACGGTAAAAC TCTGGAAATC TCCACTACAT
     1501 TCCTCTCAGC GGCTCCTCTC AATGACAGCT GAAGAAGTGA CGCGGCTGCC TGCTGTGTTT
     1561 TGATTGGTCG AATTCACTGG AGGCTTCCAG AACAGTGTAG AGTCTGAACG GGTGCGCGCT
     1621 CTGCTGTATT TAAAGGGCGA AAGAGAGACC GCAGAGAAAC TCAACCGAAG AGAAGCGACT
     1681 TGACAAAGAA GAAAAGAGCA GCCTGACAGG ACTTTTTCCC CGACGAGGTG TTTATTCGCT
     1741 CTATTTAAGA ATCTACTGTA AGGTAAGTCT CAATATATTG TACTCTATTG GCTAATCAGA
     1801 ATTATATAGA GATTATATGT ACTTAATGTC AAAAAATCAA CTTTGTATAT GTAATCTTTT
     1861 TACATGTGGA CTGCCTATGT TCATCTTATT TTAGGTCTAC TAGAAAATTA TATTTCCCGT
     1921 TTTCACAATA AGGATTTTTA AAAAAAGCAA TGAACAGACG GGCATTTACT TTATGTTGCT
     1981 GACATTATTT TATATGAGCA TAATAACCAT AAATACTAGC AAATGTCCTA AATGAATTTG
     2041 TGTTAATGTT GTCTACAAAA GAAAATTAGC GTTTTACTTG TACAACTAAT AATAACTTGG
     2101 TTATTAAGAG AATTTCACTT GTTGACTAGA AAAATCCTTT CATAATGAAA CAATTGCACC
     2161 ATAAATTGTA TAAATATAAA ATTAATTCTA ATTGTTTTTT TTTCCTGCCA AGTTTGTACA
     2221 AAAAAGTTGA ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC
     2281 ATAAAAAACA GACTACATAA TACTGTAAAA CACAACATAT GCAGTCACTA TGAACCAACT
     2341 ACTTAGATGG TATTAGTGAC CTGTAGAATT CGAGCTCTAG AGCTGCAGGG CGGCCGCGAT
     2401 ATCCCCTATA GTGAGTCGTA TTACATGGTC ATAGCTGTTT CCTGGCAGCT CTGGCCCGTG
     2461 TCTCAAAATC TCTGATGTTA CATTGCACAA GATAAAAATA TATCATCATG AACAATAAAA
     2521 CTGTCTGCTT ACATAAACAG TAATACAAGG GGTGTTATGA GCCATATTCA ACGGGAAACG
     2581 TCGAGGCCGC GATTAAATTC CAACATGGAT GCTGATTTAT ATGGGTATAA ATGGGCTCGC
     2641 GATAATGTCG GGCAATCAGG TGCGACAATC TATCGCTTGT ATGGGAAGCC CGATGCGCCA
     2701 GAGTTGTTTC TGAAACATGG CAAAGGTAGC GTTGCCAATG ATGTTACAGA TGAGATGGTC
     2761 AGACTAAACT GGCTGACGGA ATTTATGCCT CTTCCGACCA TCAAGCATTT TATCCGTACT
     2821 CCTGATGATG CATGGTTACT CACCACTGCG ATCCCCGGAA AAACAGCATT CCAGGTATTA
     2881 GAAGAATATC CTGATTCAGG TGAAAATATT GTTGATGCGC TGGCAGTGTT CCTGCGCCGG
     2941 TTGCATTCGA TTCCTGTTTG TAATTGTCCT TTTAACAGCG ATCGCGTATT TCGTCTCGCT
     3001 CAGGCGCAAT CACGAATGAA TAACGGTTTG GTTGATGCGA GTGATTTTGA TGACGAGCGT
     3061 AATGGCTGGC CTGTTGAACA AGTCTGGAAA GAAATGCATA AACTTTTGCC ATTCTCACCG
     3121 GATTCAGTCG TCACTCATGG TGATTTCTCA CTTGATAACC TTATTTTTGA CGAGGGGAAA
     3181 TTAATAGGTT GTATTGATGT TGGACGAGTC GGAATCGCAG ACCGATACCA GGATCTTGCC
     3241 ATCCTATGGA ACTGCCTCGG TGAGTTTTCT CCTTCATTAC AGAAACGGCT TTTTCAAAAA
     3301 TATGGTATTG ATAATCCTGA TATGAATAAA TTGCAGTTTC ATTTGATGCT CGATGAGTTT
     3361 TTCTAATCAG AATTGGTTAA TTGGTTGTAA CACTGGCAGA GCATTACGCT GACTTGACGG
     3421 GACGGCGCAA GCTCATGACC AAAATCCCTT AACGTGAGTT ACGCGTCGTT CCACTGAGCG
     3481 TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC
     3541 TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG
     3601 CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC
     3661 CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC
     3721 CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC
     3781 GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT
     3841 TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT
     3901 GAGCATTGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC
     3961 GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT
     4021 TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA
     4081 GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT
     4141 TGCTGGCCTT TTGCTCACAT GTT   
//