From Tol2Kit
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=== Construction details === | === Construction details === | ||
| − | pME-Gal4VP16 was made by PCR amplification of Gal4VP16 (Gal4 DNA binding domain fused to VP16 transactivation domain), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T. | + | pME-Gal4VP16 was made by PCR amplification of Gal4VP16 (Gal4 DNA binding domain fused to VP16[413-470], a partially-truncated VP16 transactivation domain), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T. |
=== Sequence === | === Sequence === | ||
Revision as of 17:15, 7 March 2010
Construction details
pME-Gal4VP16 was made by PCR amplification of Gal4VP16 (Gal4 DNA binding domain fused to VP16[413-470], a partially-truncated VP16 transactivation domain), using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, G1114T.
Sequence
Annotated sequence, Genbank format:
pME-Gal4VP16 Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
pME-Gal4VP16 sequence
Crude map
Screenshot from Sequencher showing locations of components:
