From Tol2Kit
(New page: ''Construction details'''<br> p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence, and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all ba...) |
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p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence, and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. | p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence, and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. | ||
Revision as of 19:40, 7 February 2007
Construction details
p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence, and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
p3E-IRES-EGFPCAAXpA sequence
Crude map
Screenshot from Sequencher showing locations of components:
