From Tol2Kit
(New page: '''Construction details'''<br> pDestTol2CG2 was made from pDestTol2CG by cutting with AfeII/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part o...) |
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| − | + | === Construction details === | |
pDestTol2CG2 was made from pDestTol2CG by cutting with AfeII/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality. | pDestTol2CG2 was made from pDestTol2CG by cutting with AfeII/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality. | ||
| − | + | === Crudely annotated sequence === | |
FASTA file with the full-length sequence as well as sequences of individual components):<br> | FASTA file with the full-length sequence as well as sequences of individual components):<br> | ||
[[pDestTol2CG2 sequence]] | [[pDestTol2CG2 sequence]] | ||
| − | + | === Crude map === | |
Screenshot from Sequencher showing locations of components:<br> | Screenshot from Sequencher showing locations of components:<br> | ||
[[Image:pDestTol2CG2.png]] | [[Image:pDestTol2CG2.png]] | ||
Revision as of 23:00, 19 March 2007
Construction details
pDestTol2CG2 was made from pDestTol2CG by cutting with AfeII/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2CG2 sequence
Crude map
Screenshot from Sequencher showing locations of components:
