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=== Construction details === | === Construction details === | ||
| − | p5E-''hsp70l'' was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that ''hsp70l'' is the official ZFIN gene name (thanks to Ceri van Slyke of ZFIN for pointing this out) | + | p5E-''hsp70l'' was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that ''hsp70l'' is the official ZFIN gene name (thanks to Ceri van Slyke of ZFIN for pointing this out) for the gene originally named ''hsp70'' by Halloran et al., 2000. |
Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock. | Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock. | ||
Revision as of 12:02, 1 April 2008
Construction details
p5E-hsp70l was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that hsp70l is the official ZFIN gene name (thanks to Ceri van Slyke of ZFIN for pointing this out) for the gene originally named hsp70 by Halloran et al., 2000.
Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.
Sequence
Annotated sequence, Genbank format:
p5E-hsp70l Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
p5E-hsp70l sequence
Crude map
Screenshot from Sequencher showing locations of components:
