From Tol2Kit
Construction details
pME-EGFP was made by PCR amplification of EGFP, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. Note that the EGFP sequence is followed by 60 bp of polylinker sequence from pCS2+ before the stop codon. We have confirmed functionally that this cassette produces fluorescent protein.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-EGFP sequence
Crude map
Screenshot from Sequencher showing locations of components:
