From Tol2Kit
Construction details
pME-nlsmCherry was made by PCR amplification of an nls-mCherry (mCherry with nuclear-localization signal) cassette, using primers that add att sites, followed by a BP reaction.
Sequencing finds a single PCR error in the insert, encoding an H30R mutation in mCherry. However, expression constructs made with this cassette show appropriate function (nuclear-localized fluorescence).
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-nlsmCherry sequence
Crude map
Screenshot from Sequencher showing locations of components:
