From Tol2Kit
Revision as of 02:13, 24 March 2007 by Chi-bin (talk | contribs) (New page: === Construction details === pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a...)
Construction details
pME-H2AmCherry was made by using PCR to fuse zebrafish H2A.F/Z (cloned by RT-PCR) to the N terminus of mCherry, using primers that add att sites, followed by a BP reaction. Sequencing finds a single silent mutation, A883G.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pME-H2AmCherry sequence
Crude map
Screenshot from Sequencher showing locations of components:
