From Tol2Kit
Revision as of 02:31, 24 March 2007 by Chi-bin (talk | contribs) (New page: === Construction details === p3E-polyA was made by PCR amplificaiton of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequen...)
Construction details
p3E-polyA was made by PCR amplificaiton of the SV40 late polyadenylation signal from pCS2+, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
p3E-polyA sequence
Crude map
Screenshot from Sequencher showing locations of components:
