From Tol2Kit
Construction details
p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2+MT, using primers to add att sequences, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
p3E-MTpA sequence
Crude map
Screenshot from Sequencher showing locations of components:
