From Tol2Kit
Construction details
p3E-mCherrypA was made by PCR amplification of mCherry and an SV40 late polyadenylation signal from a pCS2+-based construct, using primers to add att sites, followed by a BP reaction. Sequencing finds a single base PCR error, encoding an E7D mutation in mCherry. Functional tests of C-terminal fusion proteins made with this construct show that they yield fluorescent protein.
The mCherry sequence includes a start codon but not a good Kozak consensus (unlike p3E-EGFP-pA, which has both).
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
p3E-mCherrypA sequence
Crude map
Screenshot from Sequencher showing locations of components:
