From Tol2Kit
Construction details
pDestTol2CG2 was made from pDestTol2CG by cutting with AfeI/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2CG2 sequence
Edited 7 June 2007 to add six bases (a KpnI site that was missing).
Crude map
Screenshot from Sequencher showing locations of components:
