P3E-mCherrypA

Construction details

p3E-mCherrypA was made by PCR amplification of mCherry and an SV40 late polyadenylation signal from a pCS2+-based construct, using primers to add att sites, followed by a BP reaction. Sequencing finds a single base PCR error, encoding an E7D mutation in mCherry. Functional tests of C-terminal fusion proteins made with this construct show that they yield fluorescent protein.

The mCherry sequence includes a start codon but not a good Kozak consensus (unlike p3E-EGFP-pA, which has both).

Sequence

Annotated sequence, Genbank format:
p3E-mCherrypA Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p3E-mCherrypA sequence

Crude map

Screenshot from Sequencher showing locations of components: