From Tol2Kit
Construction details
p5E-hsp70l was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that hsp70l is the official ZFIN gene name (thanks to Ceri van Slyke of ZFIN for pointing this out); this is the same gene originally named hsp70 by Halloran et al., 2000.
Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.
Sequence
Annotated sequence, Genbank format:
p5E-hsp70l Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
p5E-hsp70l sequence
Crude map
Screenshot from Sequencher showing locations of components:
