From Tol2Kit
Construction details
pDestTol2pA2 was made from pDestTol2pA by cutting with AfeI/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
Sequence
Annotated sequence, Genbank format:
pDestTol2pA2 Genbank
FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2pA2 sequence
Edited 7 June 2007 to add six bases (a KpnI site that was missing).
Crude map
Screenshot from Sequencher showing locations of components:
