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(New page: ''Construction details'''<br> p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence, and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all ba...)
 
(Construction details)
 
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''Construction details'''<br>
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=== Construction details ===
p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence, and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.
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p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing finds a single bp change in the IRES (G481A), but this construct is functional.
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=== Sequence ===
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Annotated sequence, Genbank format:<br>
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[[p3E-IRES-EGFPCAAXpA Genbank]]
  
'''Crudely annotated sequence'''<br>
 
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
[[p3E-IRES-EGFPCAAXpA sequence]]
 
[[p3E-IRES-EGFPCAAXpA sequence]]
  
'''Crude map'''<br>
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=== Crude map ===
 
Screenshot from Sequencher showing locations of components:<br>
 
Screenshot from Sequencher showing locations of components:<br>
 
[[Image:p3E-IRES-EGFPCAAXpA.png]]
 
[[Image:p3E-IRES-EGFPCAAXpA.png]]

Latest revision as of 18:49, 17 August 2011

Construction details

p3E-IRES-EGFPCAAXpA was made by fusion PCR of the EMCV IRES sequence (IRES2 from Clontech), and EGFPCAAX-SV40 late polyA, followed by a BP reaction. Sequencing finds a single bp change in the IRES (G481A), but this construct is functional.

Sequence

Annotated sequence, Genbank format:
p3E-IRES-EGFPCAAXpA Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p3E-IRES-EGFPCAAXpA sequence

Crude map

Screenshot from Sequencher showing locations of components:
P3E-IRES-EGFPCAAXpA.png