From Tol2Kit
Jump to: navigation, search
(New page: === Construction details === p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2-MT, using primers to add att sequences, followed by a BP reaction....)
 
 
(2 intermediate revisions by the same user not shown)
Line 1: Line 1:
 
=== Construction details ===
 
=== Construction details ===
p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2-MT, using primers to add att sequences, followed by a BP reaction.  Sequencing confirms that all bases of the insert are correct.
+
p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 late polyadenylation signal from pCS2+MT, using primers to add att sequences, followed by a BP reaction.  Sequencing confirms that all bases of the insert are correct.
 +
 
 +
=== Sequence ===
 +
Annotated sequence, Genbank format:<br>
 +
[[p3E-MTpA Genbank]]
  
=== Crudely annotated sequence ===
 
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
FASTA file with the full-length sequence as well as sequences of individual components:<br>
 
[[p3E-MTpA sequence]]
 
[[p3E-MTpA sequence]]

Latest revision as of 03:52, 19 December 2007

Construction details

p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 late polyadenylation signal from pCS2+MT, using primers to add att sequences, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

Sequence

Annotated sequence, Genbank format:
p3E-MTpA Genbank

FASTA file with the full-length sequence as well as sequences of individual components:
p3E-MTpA sequence

Crude map

Screenshot from Sequencher showing locations of components:
P3E-MTpA.png