From Tol2Kit
Revision as of 03:02, 24 March 2007 by Chi-bin (talk | contribs) (Construction details)

Jump to: navigation, search

Construction details

p3E-MTpA was made by PCR-amplifying the 6x myc tag and SV40 polyadenylation signal from pCS2+MT, using primers to add att sequences, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct.

Crudely annotated sequence

FASTA file with the full-length sequence as well as sequences of individual components:
p3E-MTpA sequence

Crude map

Screenshot from Sequencher showing locations of components:
P3E-MTpA.png