From Tol2Kit
Revision as of 04:01, 19 December 2007 by Chi-bin (talk | contribs) (New page: <pre> LOCUS 228.p5E-MCS 2810 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant JM, ...)

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LOCUS       228.p5E-MCS        2810 bp ds-DNA     circular     18-DEC-2007
REFERENCE   1
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, 
			Parant JM, Yost HJ, Kanki JP, Chien CB.
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2 
			transposon transgenesis constructs.
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
  PUBMED    17937395
COMMENT     228.p5E-MCS, Tol2kit v1.0
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     668..688
                     /label=attB4
                     /ApEinfo_fwdcolor=#5d40ff
                     /ApEinfo_revcolor=#5d40ff
     misc_recomb     593..688
                     /label=attL4
                     /ApEinfo_fwdcolor=#5d40ff
                     /ApEinfo_revcolor=#5d40ff
     misc_recomb     856..1012
                     /label=attR1
                     /ApEinfo_fwdcolor=#3fd0ff
                     /ApEinfo_revcolor=#3fd0ff
     misc_feature    860..873
                     /label=att1_shared
                     /ApEinfo_fwdcolor=#32a6cc
                     /ApEinfo_revcolor=#32a6cc
     misc_feature    668..688
                     /label=att4_shared
                     /ApEinfo_fwdcolor=#4a33cc
                     /ApEinfo_revcolor=#4a33cc
     misc_feature    689..855
                     /label=pBS MCS T7-T3
                     /ApEinfo_fwdcolor=#dddddd
                     /ApEinfo_revcolor=#dddddd
     rep_origin      2134..2807
                     /label=pUC ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     CDS             1204..2013
                     /label=kanR
                     /ApEinfo_fwdcolor=#ff7f3f
                     /ApEinfo_revcolor=#ff7f3f
     primer_bind     complement(1071..1091)
                     /label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     537..552
                     /label=M13F
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        complement(836..855)
                     /label=T3
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        complement(1048..1067)
                     /label=T7
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     689..711
                     /label=T7 primer
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     terminator      268..295
                     /label=T2
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
     terminator      427..470
                     /label=T1
                     /ApEinfo_fwdcolor=#bbbbbb
                     /ApEinfo_revcolor=#bbbbbb
ORIGIN
        1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA
       61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA
      121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA
      181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC
      241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA
      301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC
      361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA
      421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG
      481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA
      541 AACGACGGCC AGTCTTAAGC TCGGGCCCGC GTTAACGCTA CCATGGAGCT CCAAATAATG
      601 ATTTTATTTT GACTGATAGT GACCTGTTCG TTGCAACAAA TTGATAAGCA ATGCTTTTTT
      661 ATAATGCCAA CTTTGTATAG AAAAGTTGGT AATACGACTC ACTATAGGGC GAATTGGGTA
      721 CCGGGCCCCC CCTCGAGGTC GACGGTATCG ATAAGCTTGA TATCGAATTC CTGCAGCCCG
      781 GGGGATCCAC TAGTTCTAGA GCGGCCGCCA CCGCGGTGGA GCTCCAGCTT TTGTTCCCTT
      841 TAGTGAGGGT TAATTCAAGT TTGTACAAAA AAGTTGAACG AGAAACGTAA AATGATATAA
      901 ATATCAATAT ATTAAATTAG ATTTTGCATA AAAAACAGAC TACATAATAC TGTAAAACAC
      961 AACATATGCA GTCACTATGA ACCAACTACT TAGATGGTAT TAGTGACCTG TAGAATTCGA
     1021 GCTCTAGAGC TGCAGGGCGG CCGCGATATC CCCTATAGTG AGTCGTATTA CATGGTCATA
     1081 GCTGTTTCCT GGCAGCTCTG GCCCGTGTCT CAAAATCTCT GATGTTACAT TGCACAAGAT
     1141 AAAAATATAT CATCATGAAC AATAAAACTG TCTGCTTACA TAAACAGTAA TACAAGGGGT
     1201 GTTATGAGCC ATATTCAACG GGAAACGTCG AGGCCGCGAT TAAATTCCAA CATGGATGCT
     1261 GATTTATATG GGTATAAATG GGCTCGCGAT AATGTCGGGC AATCAGGTGC GACAATCTAT
     1321 CGCTTGTATG GGAAGCCCGA TGCGCCAGAG TTGTTTCTGA AACATGGCAA AGGTAGCGTT
     1381 GCCAATGATG TTACAGATGA GATGGTCAGA CTAAACTGGC TGACGGAATT TATGCCTCTT
     1441 CCGACCATCA AGCATTTTAT CCGTACTCCT GATGATGCAT GGTTACTCAC CACTGCGATC
     1501 CCCGGAAAAA CAGCATTCCA GGTATTAGAA GAATATCCTG ATTCAGGTGA AAATATTGTT
     1561 GATGCGCTGG CAGTGTTCCT GCGCCGGTTG CATTCGATTC CTGTTTGTAA TTGTCCTTTT
     1621 AACAGCGATC GCGTATTTCG TCTCGCTCAG GCGCAATCAC GAATGAATAA CGGTTTGGTT
     1681 GATGCGAGTG ATTTTGATGA CGAGCGTAAT GGCTGGCCTG TTGAACAAGT CTGGAAAGAA
     1741 ATGCATAAAC TTTTGCCATT CTCACCGGAT TCAGTCGTCA CTCATGGTGA TTTCTCACTT
     1801 GATAACCTTA TTTTTGACGA GGGGAAATTA ATAGGTTGTA TTGATGTTGG ACGAGTCGGA
     1861 ATCGCAGACC GATACCAGGA TCTTGCCATC CTATGGAACT GCCTCGGTGA GTTTTCTCCT
     1921 TCATTACAGA AACGGCTTTT TCAAAAATAT GGTATTGATA ATCCTGATAT GAATAAATTG
     1981 CAGTTTCATT TGATGCTCGA TGAGTTTTTC TAATCAGAAT TGGTTAATTG GTTGTAACAC
     2041 TGGCAGAGCA TTACGCTGAC TTGACGGGAC GGCGCAAGCT CATGACCAAA ATCCCTTAAC
     2101 GTGAGTTACG CGTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT
     2161 TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA
     2221 GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC
     2281 AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC
     2341 AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT
     2401 GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG
     2461 GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC
     2521 TACACCGAAC TGAGATACCT ACAGCGTGAG CATTGAGAAA GCGCCACGCT TCCCGAAGGG
     2581 AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG
     2641 CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT
     2701 GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC
     2761 GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT 
//