From Tol2Kit
(New page: === Construction details === p5E-''hsp70'' was made by PCR amplification of the 1.5 kb hsp70 promoter, using primers to add att sites, followed by a BP reaction. Sequencing shows a single...) |
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=== Construction details === | === Construction details === | ||
| − | p5E-''hsp70'' was made by PCR amplification of the 1.5 kb hsp70 promoter, using primers to add att sites, followed by a BP reaction. | + | p5E-''hsp70'' was made by PCR amplification of the 1.5 kb hsp70 promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. |
Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock. | Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock. | ||
Revision as of 03:33, 23 March 2007
Construction details
p5E-hsp70 was made by PCR amplification of the 1.5 kb hsp70 promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
p5E-hsp70 sequence
Crude map
Screenshot from Sequencher showing locations of components:
