From Tol2Kit
m (P5E-hsp70 moved to P5E-hsp70l: Changing name to match official ZFIN gene name) |
(→Construction details) |
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=== Construction details === | === Construction details === | ||
| − | p5E-'' | + | p5E-''hsp70l'' was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that ''hsp70l'' is the official ZFIN gene name for the gene originally named ''hsp70'' by Halloran et al., 2000. Thanks to Ceri van Slyke of ZFIN for pointing this out. |
| − | Sequencing shows a single base mutation in | + | |
| + | Sequencing shows a single base mutation in the hsp70l promoter (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock. | ||
=== Sequence === | === Sequence === | ||
Annotated sequence, Genbank format:<br> | Annotated sequence, Genbank format:<br> | ||
| − | [[p5E- | + | [[p5E-hsp70l Genbank]] |
FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
| − | [[p5E- | + | [[p5E-hsp70l sequence]] |
=== Crude map === | === Crude map === | ||
Screenshot from Sequencher showing locations of components:<br> | Screenshot from Sequencher showing locations of components:<br> | ||
[[Image:p5E-hsp70.png]] | [[Image:p5E-hsp70.png]] | ||
Latest revision as of 12:03, 1 April 2008
Construction details
p5E-hsp70l was made by PCR amplification of the 1.5 kb hsp70l promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Note that hsp70l is the official ZFIN gene name for the gene originally named hsp70 by Halloran et al., 2000. Thanks to Ceri van Slyke of ZFIN for pointing this out.
Sequencing shows a single base mutation in the hsp70l promoter (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.
Sequence
Annotated sequence, Genbank format:
p5E-hsp70l Genbank
FASTA file with the full-length sequence as well as sequences of individual components:
p5E-hsp70l sequence
Crude map
Screenshot from Sequencher showing locations of components:
