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Revision as of 03:33, 23 March 2007 by Chi-bin (talk | contribs) (Construction details)

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Construction details

p5E-hsp70 was made by PCR amplification of the 1.5 kb hsp70 promoter (Genbank AF158020), using primers to add att sites, followed by a BP reaction. Sequencing shows a single base mutation in hsp70 (T1068G), but this does not appear to be a critical base as constructs made with this clone show normal induction by heatshock.

Crudely annotated sequence

FASTA file with the full-length sequence as well as sequences of individual components:
p5E-hsp70 sequence

Crude map

Screenshot from Sequencher showing locations of components:
P5E-hsp70.png