From Tol2Kit
(New page: === Construction details === pDestTol2CG was made by PCRing the cmlc2:egfp-pA cassette, adding BglII sites in the primers, then cloning into the unique BglII site of pDestTol2pA and select...) |
(→Crudely annotated sequence) |
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FASTA file with the full-length sequence as well as sequences of individual components:<br> | FASTA file with the full-length sequence as well as sequences of individual components:<br> | ||
[[pDestTol2CG sequence]] | [[pDestTol2CG sequence]] | ||
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| + | Edited 7 June 2007 to add six bases (a KpnI site that was missing). | ||
=== Crude map === | === Crude map === | ||
Screenshot from Sequencher showing locations of components:<br> | Screenshot from Sequencher showing locations of components:<br> | ||
[[Image:pDestTol2CG.png]] | [[Image:pDestTol2CG.png]] | ||
Latest revision as of 17:43, 7 June 2007
Construction details
pDestTol2CG was made by PCRing the cmlc2:egfp-pA cassette, adding BglII sites in the primers, then cloning into the unique BglII site of pDestTol2pA and selecting for reverse orientation relative to the gate and existing polyA signal. The cmlc2:egfp cassette has not been sequenced, but works in functional tests.
Crudely annotated sequence
FASTA file with the full-length sequence as well as sequences of individual components:
pDestTol2CG sequence
Edited 7 June 2007 to add six bases (a KpnI site that was missing).
Crude map
Screenshot from Sequencher showing locations of components:
