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(Construction details)
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=== Construction details ===
 
=== Construction details ===
pDestTol2CG2 was made from pDestTol2CG by cutting with AfeII/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
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pDestTol2CG2 was made from pDestTol2CG by cutting with AfeI/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
  
 
=== Crudely annotated sequence ===
 
=== Crudely annotated sequence ===

Revision as of 12:03, 11 May 2007

Construction details

pDestTol2CG2 was made from pDestTol2CG by cutting with AfeI/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.

Crudely annotated sequence

FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2CG2 sequence

Crude map

Screenshot from Sequencher showing locations of components:
PDestTol2CG2.png