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=== Construction details ===
 
=== Construction details ===
pDestTol2CG2 was made from pDestTol2CG by cutting with AfeII/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
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pDestTol2CG2 was made from pDestTol2CG by cutting with AfeI/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
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=== Sequence ===
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Annotated sequence, Genbank format:<br>
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[[pDestTol2CG2 Genbank]]
  
=== Crudely annotated sequence ===
 
 
FASTA file with the full-length sequence as well as sequences of individual components):<br>
 
FASTA file with the full-length sequence as well as sequences of individual components):<br>
 
[[pDestTol2CG2 sequence]]
 
[[pDestTol2CG2 sequence]]
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Edited 7 June 2007 to add six bases (a KpnI site that was missing).
  
 
=== Crude map ===
 
=== Crude map ===
 
Screenshot from Sequencher showing locations of components:<br>
 
Screenshot from Sequencher showing locations of components:<br>
 
[[Image:pDestTol2CG2.png]]
 
[[Image:pDestTol2CG2.png]]

Latest revision as of 03:55, 19 December 2007

Construction details

pDestTol2CG2 was made from pDestTol2CG by cutting with AfeI/NgoMI and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.

Sequence

Annotated sequence, Genbank format:
pDestTol2CG2 Genbank

FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2CG2 sequence

Edited 7 June 2007 to add six bases (a KpnI site that was missing).

Crude map

Screenshot from Sequencher showing locations of components:
PDestTol2CG2.png