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(Crudely annotated sequence)
 
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pDestTol2pA2 was made from pDestTol2pA by cutting with AfeI/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
 
pDestTol2pA2 was made from pDestTol2pA by cutting with AfeI/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.
  
=== Crudely annotated sequence ===
+
=== Sequence ===
 +
Annotated sequence, Genbank format:<br>
 +
[[pDestTol2pA2 Genbank]]
 +
 
 
FASTA file with the full-length sequence as well as sequences of individual components):<br>
 
FASTA file with the full-length sequence as well as sequences of individual components):<br>
 
[[pDestTol2pA2 sequence]]
 
[[pDestTol2pA2 sequence]]

Latest revision as of 03:55, 19 December 2007

Construction details

pDestTol2pA2 was made from pDestTol2pA by cutting with AfeI/DraIII and religating; this removed ~2kb comprising medaka tyrosinase genomic sequence and part of the f1 ori. This reduced the size of the construct without affecting its functionality.

Sequence

Annotated sequence, Genbank format:
pDestTol2pA2 Genbank

FASTA file with the full-length sequence as well as sequences of individual components):
pDestTol2pA2 sequence

Edited 7 June 2007 to add six bases (a KpnI site that was missing).

Crude map

Screenshot from Sequencher showing locations of components:
PDestTol2pA2.png