From Tol2Kit
Revision as of 04:12, 19 December 2007 by Chi-bin (talk | contribs) (New page: <pre> LOCUS 394.pDestTol2pA2 5883 bp ds-DNA circular 18-DEC-2007 REFERENCE 1 AUTHORS Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, Parant...)

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LOCUS       394.pDestTol2pA2        5883 bp ds-DNA     circular     18-DEC-2007
REFERENCE   1
  AUTHORS   Kwan KM, Fujimoto E, Grabher C, Mangum BD, Hardy ME, Campbell DS, 
			Parant JM, Yost HJ, Kanki JP, Chien CB.
  TITLE     The Tol2kit: a multisite gateway-based construction kit for Tol2 
			transposon transgenesis constructs.
  JOURNAL   Developmental Dynamics 236(11):3088-99 (2007).
  PUBMED    17937395
COMMENT     394.pDestTol2pA2, Tol2kit v1.0
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_recomb     2676..2800
                     /label=attR3
                     /ApEinfo_fwdcolor=#5370ff
                     /ApEinfo_revcolor=#5370ff
     misc_recomb     1097..1221
                     /label=attR4
                     /ApEinfo_fwdcolor=#5d40ff
                     /ApEinfo_revcolor=#5d40ff
     misc_feature    2780..2794
                     /label=att3_shared
                     /ApEinfo_fwdcolor=#4259cc
                     /ApEinfo_revcolor=#4259cc
     misc_feature    1097..1117
                     /label=att4_shared
                     /ApEinfo_fwdcolor=#4a33cc
                     /ApEinfo_revcolor=#4a33cc
     polyA_signal    2840..3037
                     /label=SV40 late polyA (from pCS2+)
                     /ApEinfo_fwdcolor=#ffff7f
                     /ApEinfo_revcolor=#ffff7f
     rep_origin      complement(4081..4754)
                     /label=pUC ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     rep_origin      complement(4122..4804)
                     /label=colE1 ori
                     /ApEinfo_fwdcolor=#999999
                     /ApEinfo_revcolor=#999999
     CDS             complement(4902..5759)
                     /label=ampR
                     /ApEinfo_fwdcolor=#ff7f00
                     /ApEinfo_revcolor=#ff7f00
     CDS             complement(1908..2567)
                     /label=cmR
                     /ApEinfo_fwdcolor=#ffbf00
                     /ApEinfo_revcolor=#ffbf00
     misc_feature    complement(1261..1566)
                     /label=ccdB
                     /ApEinfo_fwdcolor=#ffbf3f
                     /ApEinfo_revcolor=#ffbf3f
     primer_bind     1061..1082
                     /label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     complement(3733..3754)
                     /label=M13R(1)
                     /ApEinfo_label=M13R
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     primer_bind     complement(2809..2824)
                     /label=M13F
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     promoter        complement(3698..3717)
                     /label=T3
                     /ApEinfo_fwdcolor=#777777
                     /ApEinfo_revcolor=#777777
     LTR             complement(514..530)
                     /label=Tol2 3' TIR
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     LTR             complement(3594..3612)
                     /label=Tol2 5' TIR
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=#ff0000
     misc_structure  complement(3096..3430)
                     /label=Tol2 exon1 bp1-335 of 461
                     /ApEinfo_fwdcolor=#aa0000
                     /ApEinfo_revcolor=#aa0000
     misc_structure  complement(703..1049)
                     /label=Tol2 exon4 136-482
                     /ApEinfo_fwdcolor=#aa0000
                     /ApEinfo_revcolor=#aa0000
     misc_structure  complement(3613..3649)
                     /label=medaka tyrosinase ex5 frag
                     /ApEinfo_fwdcolor=#006600
                     /ApEinfo_revcolor=#006600
     gene            228..514
                     /label=medaka tyrosinase genomic (from AB010101)
                     /ApEinfo_fwdcolor=#006600
                     /ApEinfo_revcolor=#006600
ORIGIN
        1 CCACCTAAAT TGTAAGCGTT AATATTTTGT TAAAATTCGC GTTAAATTTT TGTTAAATCA
       61 GCTCATTTTT TAACCAATAG GCCGAAATCG GCAAAATCCC TTATAAATCA AAAGAATAGA
      121 CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGAACAAGAG TCCACTATTA AAGAACGTGG
      181 ACTCCAACGT CAAAGGGCGA AAAACCGTCT ATCAGGGCGA TGGCCCACGC TGGCTAAGAA
      241 CTCATCAGCC TCCCCGGTCC ATCTACCCAC GTACCAATGC ACCAATTGGC CACAATGACG
      301 GCTACTACAT GGTGCCATTC CTTCCTCTTT ATAGGAATGG AGACTACCTC CTGTCCAACA
      361 AGGCTCTTGG ATACGAGTAC GCCTACCTGT TGGACCCAGG TCATTGCACA ACACCAGAAA
      421 TGCCCTCTGA TCTGCAAAAG ACGTGAATAT CTGTTCAGAC ACCCATATCC ACTCTGTTCC
      481 ACACAGGTCA GAGGTTTGTC CAGGAGTTCT TGACAGAGGT GTAAAAAGTA CTCAAAAATT
      541 TTACTCAAGT GAAAGTACAA GTACTTAGGG AAAATTTTAC TCAATTAAAA GTAAAAGTAT
      601 CTGGCTAGAA TCTTACTTGA GTAAAAGTAA AAAAGTACTC CATTAAAATT GTACTTGAGT
      661 ATTAAGGAAG TAAAAGTAAA AGCAAGAAAG AAAACTAGAG ATTCTTGTTT AAGCTTTTAA
      721 TCTCAAAAAA CATTAAATGA AATGCATACA AGGTTTTATC CTGCTTTAGA ACTGTTTGTA
      781 TTTAATTATC AAACTATAAG ACAGACAATC TAATGCCAGT ACACGCTACT CAAAGTTGTA
      841 AAACCTCAGA TTTAACTTCA GTAGAAGCTG ATTCTCAAAA TTGTTAGTGT CAAGCCTAGC
      901 TCTTTTGGGG CTGAAAAGCA ATCCTGCAGT GCTGAAAAGC CTCTCACAGG CAGCCGATGC
      961 GGGAAGAGGT GTATTAGTCT TGATAGAGAG GCTGCAAATA GCAGGAAACG TGAGCAGAGA
     1021 CTCCCTGGTG TCTGAAACAC AGGCCAGATG GGCCCTCGAG CAGGAAACAG CTATGACCAT
     1081 GATTACGCCA AGCTATCAAC TTTGTATAGA AAAGTTGAAC GAGAAACGTA AAATGATATA
     1141 AATATCAATA TATTAAATTA GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA
     1201 CAACATATCC AGTCACTATG GTCGACCTGC AGACTGGCTG TGTATAAGGG AGCCTGACAT
     1261 TTATATTCCC CAGAACATCA GGTTAATGGC GTTTTTGATG TCATTTTCGC GGTGGCTGAG
     1321 ATCAGCCACT TCTTCCCCGA TAACGGAGAC CGGCACACTG GCCATATCGG TGGTCATCAT
     1381 GCGCCAGCTT TCATCCCCGA TATGCACCAC CGGGTAAAGT TCACGGGGGA CTTTATCTGA
     1441 CAGCAGACGT GCACTGGCCA GGGGGATCAC CATCCGTCGC CCGGGCGTGT CAATAATATC
     1501 ACTCTGTACA TCCACAAACA GACGATAACG GCTCTCTCTT TTATAGGTGT AAACCTTAAA
     1561 CTGCATTTCA CCAGCCCCTG TTCTCGTCGG CAAAGGAGCC GTTCATTTCA ATAAACCGGG
     1621 CGACCTCAGC CATCCCTTCC TGATTTTCCG CTTTCCAGCG TTCGGCACGC AGACGACGGG
     1681 CTTCATTCTG CATGGTTGTG CTTACCGAAC CGGAGATATT GACATCATAT ATGCCTTGAG
     1741 CAACTGATAG CTGTCGCTGT CAACTGTCAC TGTAATACGC TGCTTCATAG CATACCTCTT
     1801 TTTGACATAC TTCGGGTATA CATATCAGTA TATATTCTTA TACCGCAAAA ATCAGCGCGC
     1861 AAATACGCAT ACTGTTATCT GGCTTTTAGT AAGCCGGATC CTCTAGATTA CGCCCCGCCC
     1921 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA
     1981 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA
     2041 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA
     2101 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT
     2161 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA
     2221 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA
     2281 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT
     2341 GCCATACGGA ATTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA
     2401 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG
     2461 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC
     2521 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATTTT AGCTTCCTTA
     2581 GCTCCTGAAA ATCTCGACGG ATCCTAACTC AAAATCCACA CATTATACGA GCCGGAAGCA
     2641 TAAAGTGTAA AGCCTGGGGG TGCCTAATGC GGCCGCCATA GTGACTGGAT ATGTTGTGTT
     2701 TTACAGTATT ATGTAGTCTG TTTTTTATGC AAAATCTAAT TTAATATATT GATATTTATA
     2761 TCATTTTACG TTTCTCGTTC AACTTTATTA TACATAGTTG ATAATTCACT GGCCGTCGTT
     2821 TTACGGTACC ATCGATGATG ATCCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC
     2881 CACAACTAGA ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT
     2941 ATTTGTAACC ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT
     3001 GTTTCAGGTT CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG
     3061 TGGTATGGCT GATTATGATC CTCTAGATCA GATCTGCGAA GATACGGCCA CGGGTGCTCT
     3121 TGATCCTGTG GCTGATTTTG GACTGTGCTG CTCGCAGCTG CTGATGAATC ACATACTTCC
     3181 TCCATTTTCT TCCACTGATT GACTGTTATA ATTTCCCTAA TTTCCAGGTC AAGGTGCTGT
     3241 GCATTGTGGT AATAGATGTG ACATGACGTC ACTTCCAAAG GACCAATGAA CATGTCTGAC
     3301 CAATTTCATA TAATGTGAAA ACGATTTTCA TAGGCAGAAT AAATAACATT TAAATTAAAC
     3361 TGGGCATCAG CGCAATTCAA TTGGTTTGGT AATAGCAAGG GAAAATAGAA TGAAGTGATC
     3421 TCCAAAAAAT AAGTACTTTT TGACTGTAAA TAAAATTGTA AGGAGTAAAA AGTACTTTTT
     3481 TTTCTAAAAA AATGTAATTA AGTAAAAGTA AAAGTATTGA TTTTTAATTG TACTCAAGTA
     3541 AAGTAAAAAT CCCCAAAAAT AATACTTAAG TACAGTAATC AAGTAAAATT ACTCAAGTAC
     3601 TTTACACCTC TGGTTCTTGA CCCCCTACCT TCAGCAAGCC CAGCAGATCC ACTAGTTCTA
     3661 GAGCGGCCGC CACCGCGGTG GAGCTCCAGC TTTTGTTCCC TTTAGTGAGG GTTAATTGCG
     3721 CGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT
     3781 CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC
     3841 TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC
     3901 CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT
     3961 TCCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA
     4021 GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC
     4081 ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT
     4141 TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG
     4201 CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC
     4261 TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC
     4321 GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC
     4381 AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC
     4441 TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT
     4501 AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT
     4561 AACTACGGCT ACACTAGAAG GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC
     4621 TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT
     4681 TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG
     4741 ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC
     4801 ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA
     4861 TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG
     4921 GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG
     4981 TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA
     5041 GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG
     5101 CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA
     5161 GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC
     5221 ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA
     5281 AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG
     5341 ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT
     5401 AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC
     5461 AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG
     5521 GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG
     5581 GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT
     5641 GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA
     5701 GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA
     5761 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC
     5821 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
     5881 GTG     
//