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Revision as of 01:38, 24 March 2007 by Chi-bin (talk | contribs) (Construction details)

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Construction details

pME-EGFP was made by PCR amplification of EGFP, using primers that add att sites, followed by a BP reaction. Sequencing confirms that all bases of the insert are correct. Note that the EGFP sequence is followed by 60 bp of polylinker sequence from pCS2+ before the stop codon. We have confirmed functionally that this cassette produces fluorescent protein.

Crudely annotated sequence

FASTA file with the full-length sequence as well as sequences of individual components:
pME-EGFP sequence

Crude map

Screenshot from Sequencher showing locations of components:
PME-EGFP.png